Thiol-Capped Gold Nanoparticles Swell-Encapsulated into Polyurethane as Powerful Antibacterial Surfaces Under Dark and Light Conditions
Thiol-Capped Gold Nanoparticles Swell-Encapsulated into Polyurethane as Powerful Antibacterial Surfaces Under Dark and Light Conditions
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DOI：
10.1038/srep39272
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发表时间：
2016-12-16
影响因子：
4.6
通讯作者：
Parkin, Ivan P.
中科院分区：
文献类型：
--
作者：
Macdonald, Thomas J.;Wu, Ke;Parkin, Ivan P.
展示了一种使用硫醇封顶的金纳米颗粒(AuNPs)开发抗菌表面的简单程序，它可以在黑暗和光照条件下有效地杀灭细菌。报道了AuNP的大小和浓度对光活化抗菌表面的影响，结果表明，结晶紫(CV)涂层的聚氨酯具有显著的尺寸效应和杀菌活性。这些材料已被证明是强大的抗菌表面，既可以对抗革兰氏阳性菌，也可以对抗革兰氏阴性菌。将直径为2、3或5 nm的AuNPs溶胀包覆到PU中，然后涂覆一层CV(称为PU-AuNPs-CV)。在黑暗和光照条件下测试了PU-AuNPs-CV样品对具有代表性的革兰氏阳性和革兰氏阴性杆菌金黄色葡萄球菌和大肠杆菌的抗菌活性。本研究中的所有光照条件都模拟了一个典型的白光医院环境。这项工作表明，PU-AuNPs-CV样品的抗菌活性和三芳基甲烷类染料的光活性的协同增强高度依赖于纳米粒子的尺寸和浓度。当2 nm AuNPs的溶胀包埋浓度为1.0 mg mL(-1)时，PU-AuNPs-CV的抗菌效果最好。两小时后，在黑暗和光照条件下，革兰氏阳性和革兰氏阴性细菌都降到了检测下限(>；4log)以下。
A simple procedure to develop antibacterial surfaces using thiol-capped gold nanoparticles (AuNPs) is shown, which effectively kill bacteria under dark and light conditions. The effect of AuNP size and concentration on photo-activated antibacterial surfaces is reported and we show significant size effects, as well as bactericidal activity with crystal violet (CV) coated polyurethane. These materials have been proven to be powerful antibacterial surfaces against both Gram-positive and Gram-negative bacteria. AuNPs of 2, 3 or 5 nm diameter were swell-encapsulated into PU before a coating of CV was applied (known as PU-AuNPs-CV). The antibacterial activity of PU-AuNPs-CV samples was tested against Staphylococcus aureus and Escherichia coli as representative Gram-positive and Gram-negative bacteria under dark and light conditions. All light conditions in this study simulated a typical white-light hospital environment. This work demonstrates that the antibacterial activity of PU-AuNPs-CV samples and the synergistic enhancement of photoactivity of triarylmethane type dyes is highly dependent on nanoparticle size and concentration. The most powerful PU-AuNPs-CV antibacterial surfaces were achieved using 1.0 mg mL(-1) swell encapsulation concentrations of 2 nm AuNPs. After two hours, Gram-positive and Gram-negative bacteria were reduced to below the detection limit (>4 log) under dark and light conditions.