Ovulation response and fertilization failure in immature rats induced to superovulate.
Ovulation response and fertilization failure in immature rats induced to superovulate.
复制标题
诱导超排卵的未成熟大鼠的排卵反应和受精失败。
DOI：
10.1530/jrf.0.0670091
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. Armstrong
中科院分区：
文献类型：
--
作者：
E. Walton;G. Evans;D. Armstrong
未成熟雌性大鼠（75 g体重，29日龄）注射4或40 i.u. PMSG，并在第-2天18：00 h至第1天09：00 h之间的时间间隔处死。对照动物（4 i.u.）在第1天00：30和05：30之间排卵，而从超排卵大鼠（40 i.u. PMSG）在第-1天06：00 h至第0天24：00 h之间缓慢增加，在第0天24：00至第1天06：00之间显著增加。在第0天，将经类似处理的大鼠与经证实具有生育力的雄性大鼠一起笼养过夜，并在第1天14：00至16：00 h之间处死。与对照动物相比，从超数排卵大鼠中回收的正常1-细胞卵子的百分比显著较低（71.6%和98.5%），并且这些1-细胞卵子的受精百分比较低（69.7%和99.1%）。在对照组中，所有交配动物的受精卵比例都很高，而26%的超排卵大鼠没有受精或受精比例很低。在对照组动物中，有精子穿透和原核形成的证据;在超数排卵大鼠中，具有原核的卵子明显少于穿透的卵子。这些结果表明，超数排卵的未成熟大鼠的生育能力下降是由于在某些动物中完全或部分受精失败。排卵期的延长可能是一个促成因素。
Immature female rats (75 g body wt, aged 29 days) were injected with 4 or 40 i.u. PMSG on Day -2 and were killed at intervals between 18:00 h on Day -2 and 09:00 h on Day 1. Control animals (4 i.u.) ovulated between 00:30 and 05:30 h on Day 1 whereas the number of ova recovered from superovulated rats (40 i.u. PMSG) increased slowly between 06:00 h on Day -1 and 24:00 h on Day 0 and markedly between 24:00 on Day 0 and 06:00 on Day 1. Similarly treated rats were caged overnight on Day 0 with males of proven fertility and killed between 14:00 and 16:00 h on Day 1. A significantly lower percentage of normal 1-cell ova was recovered from the superovulated rats compared to control animals (71.6 and 98.5%) and of these 1-cell ova a lower percentage was fertilized (69.7 and 99.1%). In the control group all mated animals had a high proportion of ova fertilized whereas 26% of superovulated rats had none or a very low proportion fertilized. In the control animals there was evidence of sperm penetration and pronucleus formation; in superovulated rats significantly fewer ova had pronuclei than were penetrated. These results suggest that reduced fertility of superovulated immature rats is due to complete or partial failure of fertilization in some animals. The extended period during which ovulation occurs may be a contributory factor.