Preparation of fatty acid methyl esters for gas-liquid chromatography
Preparation of fatty acid methyl esters for gas-liquid chromatography
复制标题
DOI：
10.1194/jlr.d001065
复制
发表时间：
2010-03-01
影响因子：
6.5
通讯作者：
Fukubayashi, Yumeto
中科院分区：
文献类型：
--
作者：
Ichihara, Ken'ichi;Fukubayashi, Yumeto
以工业浓盐酸（35%w/w）为酸催化剂，研究了由甾醇酯、甘油三酯、磷脂和脂肪酸制备脂肪酸甲酯（FAMEs）的方法。用41.5 ml甲醇稀释9.7 ml浓HCl，制备8%（w/v）HCl甲醇/水（85：15，v/v）溶液。将甲苯（0.2ml）、甲醇（1.5ml）和8%HCl溶液（0.3ml）依次加入脂质样品中。最终HCl浓度为1.2%（w/v）。将该溶液（2 ml）在45 ℃下孵育过夜或在100 ℃下加热1-1.5小时。对于上述脂质类别，在存在源自浓HCl的水的情况下形成的FFA的量估计为96%，并且与通过皂化/甲基化或通过使用商业无水HCl/甲醇的酸催化甲醇分解/甲基化获得的FFA的量相同或更好。本文开发的方法可以成功地应用于各种脂质样品的脂肪酸分析，包括鱼油，植物油和血脂的GC分析。Ichihara，K.，和Y.小林气液色谱用脂肪酸甲酯的制备。J. Lipid Res. 2010. 51：635-640。
A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol. Toluene (0.2 ml), methanol (1.5 ml), and the 8% HCl solution (0.3 ml) were added sequentially to the lipid sample. The final HCl concentration was 1.2% (w/v). This solution (2 ml) was incubated at 45 degrees C overnight or heated at 100 degrees C for 1-1.5 h. The amount of FFA formed in the presence of water derived from conc. HCl was estimated to be 96% for the above lipid classes and were the same as or better than those obtained by saponification/methylation or by acid-catalyzed methanolysis/methylation using commercial anhydrous HCl/methanol. The method developed here could be successfully applied to fatty acid analysis of various lipid samples, including fish oils, vegetable oils, and blood lipids by GC.-Ichihara, K., and Y. Fukubayashi. Preparation of fatty acid methyl esters for gas-liquid chromatography. J. Lipid Res. 2010. 51: 635-640.