Functional characterization of middle ear mucosa residues in cholesteatoma samples.
Functional characterization of middle ear mucosa residues in cholesteatoma samples.
复制标题
胆脂瘤样品中中耳粘膜残留物的功能表征。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
A. Fisseler‐Eckhoff
中科院分区：
文献类型：
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作者：
H. Sudhoff;J. Bujía;A. Holly;C. Kim;A. Fisseler‐Eckhoff
胆脂瘤上皮的特征是角质形成细胞失调，其侵袭性生长导致正常中耳粘膜的破坏。中耳炎上皮细胞的异常行为似乎是由大量免疫细胞浸润引起的，大量释放不同的细胞因子和生长因子。中耳粘膜残端常位于中耳乳突瘤间质内或邻近中耳乳突瘤上皮的前缘。本研究调查了白细胞介素-1（IL-1）、转化生长因子-α（TGF-α）、表皮生长因子（EGF）和表皮生长因子受体（EGF-R）在粘膜剩余物中的存在以及活化标记物4F 2的表达。这些发现与周围间质的特征相关，免疫细胞浸润增强。胆脂瘤上皮显示IL-1、TGF-α和EGF-R的高染色强度。与此相反，中耳粘膜对上述因子未显示任何阳性反应。胆脂瘤上皮和中耳粘膜残留物中均未发现表皮生长因子免疫反应性。作者在周围基质中发现了高浓度的淋巴细胞和巨噬细胞。这些细胞中的大多数表达TGF-α、IL-1和4F 2，表明为活化形式。结果表明，存在于中耳粘膜中的角质形成细胞似乎对发炎基质释放的刺激没有反应，这反映了形成两种类型上皮的角质形成细胞的细胞生物学特征的重要差异。
Cholesteatoma epithelium is characterized by a keratinocyte dysregulation with an aggressive growth that leads to the destruction of normal middle ear mucosa. The abnormal behavior of cholesteatoma epithelium seems to be induced by the presence of a heavy immune cell infiltrate releasing different cytokines and growth factors in high amounts. Middle ear mucosa rests are often observed within the cholesteatoma stroma or adjacent to the advancing front of cholesteatoma epithelium. This study investigated the presence of interleukin-1 (IL-1), transforming growth factor-alpha (TGF-alpha), epidermal growth factor (EGF), and epidermal growth factor-receptor (EGF-R) in the mucosa rests as well as the expression of an activation marker, 4F2. The findings were correlated with the features of a surrounding stroma with an enhanced immune cell infiltrate. Cholesteatoma epithelium showed a high staining intensity of IL-1, TGF-alpha, and EGF-R. In contrast to this, middle ear mucosa did not show any positive reactions for the mentioned factors. Epidermal growth factor immunoreactivity was found in neither cholesteatoma epithelium nor in middle ear mucosa residues. The authors found a high concentration of lymphocytes and macrophages in the surrounding stroma. Most of these cells expressed TGF-alpha, IL-1, and 4F2, suggesting an activated form. Results indicate that keratinocytes present in the middle ear mucosa do not appear to react to the stimuli released by the inflamed stroma, reflecting important differences in the cell biological features of the keratinocytes that form parts of both types of epithelium.