Tricarboxylic acid cycle activity measured by 13C magnetic resonance spectroscopy in rats subjected to the kaolin model of obstructed hydrocephalus.
Tricarboxylic acid cycle activity measured by 13C magnetic resonance spectroscopy in rats subjected to the kaolin model of obstructed hydrocephalus.
复制标题
通过受阻脑积水的高岭土模型，通过13C磁共振光谱测量的三羧酸循环活性。
DOI：
10.1007/s11064-011-0497-z
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发表时间：
2011-10
影响因子：
4.4
通讯作者：
Sonnewald, Ursula
中科院分区：
文献类型：
--
作者：
Melo, Torun M.;Haberg, Asta K.;Risa, Oystein;Kondziella, Daniel;Henry, Pierre-Gilles;Sonnewald, Ursula
Evaluating early changes in cerebral metabolism in hydrocephalus can help in the decision making and the timing of surgical intervention. This study was aimed at examining the tricarboxylic acid (TCA) cycle rate and 13C label incorporation into neurotransmitter amino acids and other compounds 2 weeks after rats were subjected to kaolin-induced progressive hydrocephalus. In vivo and ex vivo magnetic resonance spectroscopy (MRS), combined with the infusion of [1,6-13C]glucose, was used to monitor the time courses of 13C label incorporation into the different carbon positions of glutamate in the forebrains of rats with hydrocephalus as well as in those of controls. Metabolic rates were determined by fitting the measured data into a one-compartment metabolic model. The TCA cycle rate was 1.3 ± 0.2 μmoles/gram/minute in the controls and 0.8 ± 0.4 μmoles/gram/minute in the acute hydrocephalus group, the exchange rate between α-ketoglutarate and glutamate was 4.1 ± 2.5 μmoles/gram/minute in the controls and 2.7 ± 2.6 μmoles/gram/minute in the hydrocephalus group calculated from in vivo MRS. There were no statistically significant differences between these rates. Hydrocephalus caused a decrease in the amounts of glutamate, alanine and taurine. In addition, the concentration of the neuronal marker N-acetyl aspartate was decreased. 13C Labelling of most amino acids derived from [1,6-13C]glucose was unchanged 2 weeks after hydrocephalus induction. The only indication of astrocyte impairment was the decreased 13C enrichment in glutamine C-2. This study shows that hydrocephalus causes subtle but significant alterations in neuronal metabolism already early in the course of the disease. These sub-lethal changes, however, if maintained and if ongoing might explain the delayed and programmed neuronal damage as seen in chronic hydrocephalus.
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影响因子：
4.1
作者：
Braun, KPJ;van Eijsden, P;Nicolay, K
通讯作者：
Nicolay, K
影响因子：
4.7
作者：
GEDDES, JW;WOOD, JD
通讯作者：
WOOD, JD
影响因子：
4.7
作者：
Kondziella, D;Qu, H;Sonnewald, U
通讯作者：
Sonnewald, U
影响因子：
3.3
作者：
GRUETTER, R
通讯作者：
GRUETTER, R
DOI：
10.1007/bf01860670
发表时间：
1932-01-01
影响因子：
--
作者：
Dixon, WE;Heller, H
通讯作者：
Heller, H