Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae.
Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae.
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DOI：
10.1091/mbc.e09-04-0345
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发表时间：
2010-07-01
影响因子：
3.3
通讯作者：
Reggiori F
中科院分区：
文献类型：
--
作者：
van der Vaart A;Griffith J;Reggiori F
通过自噬将蛋白质和细胞器运输到液泡的过程涉及膜重排，从而导致自噬体的形成。我们研究了高尔基体在自噬中的作用，发现，在酵母中，该细胞器在为自噬体生物发生提供所需的脂质双层方面起着至关重要的作用。
通过自噬将蛋白质和细胞器运输到液泡的过程涉及膜重排，从而导致被称为自噬体的大型囊泡的形成。自噬体生物发生的潜在机制以及构成这些囊泡的膜的来源在很大程度上仍不清楚。我们研究了高尔基体复合物在自噬中的作用，并确定在酵母中，Sec7、Gea1和Gea2对ADP - 核糖基化因子（Arf）1和Arf2 GTP酶的激活对这种分解代谢过程至关重要。这些成分催化的两个主要事件，即COPI包被囊泡和网格蛋白包被囊泡的生物发生，在自噬中并不起关键作用。对饥饿条件下sec7菌株的分析表明，自噬机制正确组装，自噬体的前体膜池（即吞噬泡）正常形成。然而，吞噬泡向自噬体的扩张受到严重损害。我们的数据表明，高尔基体复合物在为双层膜囊泡的生物发生提供所需的脂质双层方面起着至关重要的作用，可能是通过一类新的运输载体或一种新的机制。
The delivery of proteins and organelles to the vacuole by autophagy involves membrane rearrangements that result in the formation of autophagosomes. We have investigated the role of the Golgi in autophagy and found that, in yeast, this organelle plays a crucial role in supplying lipid bilayers necessary for autophagosome biogenesis. The delivery of proteins and organelles to the vacuole by autophagy involves membrane rearrangements that result in the formation of large vesicles called autophagosomes. The mechanism underlying autophagosome biogenesis and the origin of the membranes composing these vesicles remains largely unclear. We have investigated the role of the Golgi complex in autophagy and have determined that in yeast, activation of ADP-ribosylation factor (Arf)1 and Arf2 GTPases by Sec7, Gea1, and Gea2 is essential for this catabolic process. The two main events catalyzed by these components, the biogenesis of COPI- and clathrin-coated vesicles, do not play a critical role in autophagy. Analysis of the sec7 strain under starvation conditions revealed that the autophagy machinery is correctly assembled and the precursor membrane cisterna of autophagosomes, the phagophore, is normally formed. However, the expansion of the phagophore into an autophagosome is severely impaired. Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism.