Serum alpha‐fetoprotein in rats after ligation of the common bile duct: Relation to ductular cell (oval cell) proliferation
Serum alpha‐fetoprotein in rats after ligation of the common bile duct: Relation to ductular cell (oval cell) proliferation
复制标题
胆总管结扎后大鼠血清甲胎蛋白：与导管细胞（卵圆细胞）增殖的关系
作者：
Wu Pui Chee;Lily T Ma;J. Gibson;H. Hirai;Y. Tsukada
用放射免疫法测定大鼠胆总管结扎、切断后不同时期及假结扎后血清甲胎蛋白（AFP）水平。在胆总管结扎后3 ~ 10天、17天和28天，血清AFP短暂升高，达到统计学显著水平，而假手术大鼠则无此现象。放射自显影法测定，血清AFP的峰值升高发生在胆管细胞和肝细胞的有丝分裂波之后。然而，肝细胞的增殖与小管细胞的增殖相比仍然很小。肝脏的超微结构和组织化学研究清楚地区分了导管（卵圆）细胞和肝细胞，没有提供从导管细胞向肝细胞转变的证据。我们的研究结果表明，胆管结扎诱导的大鼠胆管细胞增殖可能导致血清AFP升高。
Serum AFP levels were measured by radio-immunoassay in rats at various periods after ligation and section of the common bile duct and after sham-ligation. Transient elevations of serum AFP reaching statistically significant levels occurred at 3 to 10 days, 17 days and 28 days after ligation of the common bile duct but not in sham-operated rats. The peak elevations of serum AFP followed waves of mitosis in both the ductular cells and the hepatocytes as measured by autoradiography. Proliferation of hepatocytes, however, remained minor when compared to ductular cell multiplication. Ultra-structural and histochemical studies of the liver clearly distinguished ductular (oval) cells from hepatocytes and provided no evidence of transition from ductular cells to hepatocytes. Our findings suggest that ductular cell proliferation in the rat induced by ligation of the common bile duct may produce elevations in the serum AFP.