Survivin, bcl-2, bax, and bcl-X gene expression in sentinel lymph nodes from melanoma patients
Survivin, bcl-2, bax, and bcl-X gene expression in sentinel lymph nodes from melanoma patients
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DOI：
10.1200/jco.2003.08.066
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发表时间：
2003-01-15
影响因子：
45.3
通讯作者：
Aglianò, AM
中科院分区：
文献类型：
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作者：
Gradilone, A;Gazzaniga, P;Aglianò, AM
目的：利用逆转录聚合酶链式反应（RT-PCR）和免疫组化技术检测黑色素瘤前哨淋巴结（sln）中survivin、bcl-2、bcl-X、bax等凋亡相关基因的表达，并将其与患者预后进行相关性分析。患者和方法：对36例sln进行了检查。RNA提取后，采用RT-PCR和Southern blot杂交检测survivin、bci-2、bcl-X和box mRNA。Bci-2、survivin和box基因的表达在可能的情况下也通过免疫组织化学在蛋白水平上进行评估。结果：survivin表达与患者预后有显著相关性（P < 0.005）；事实上，61.5%的表达survivin基因的患者因疾病进展或死亡，而38.5%的患者目前无病。在survivin表达阴性的患者中，100%的患者在中位随访52.9个月后无病。我们没有发现bcl-2、box和bcl-X基因表达与患者预后之间的显著相关性。事实上，这些基因在疾病进展患者和无疾病患者中表达相同。结论：我们的研究结果表明，在黑色素瘤患者的sln中，凋亡相关基因的表达是可变的；更有趣的是，我们发现survivin的表达与患者的预后有统计学意义上的相关性，而其他基因，如bcl-2、box和bcl-X的表达似乎与疾病的进展无关。我们认为通过RT-PCR检测sln中survivin基因的表达可能是一个有用的预后指标。(C) 2003年由美国临床肿瘤学会出版。
Purpose : The expression of apoptosis-related genes, such as survivin, bcl-2, bcl-X, and bax, has been evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and by immunohistochemistry in sentinel lymph nodes (SLNs) from melanoma patients and then correlated to the outcome of patients.Patients and Methods: Thirty-six SLNs were examined. After RNA extraction, an RT-PCR followed by Southern blot hybridization was performed to detect survivin, bci-2, bcl-X, and box mRNA. bci-2, survivin, and box gene expression was evaluated, whenever possible, also by immunohistochemistry at the protein level.Results: We found a significant correlation (P < .005) between survivin expression and outcome of patients; in fact, 61.5% of patients expressing survivin gene progressed or died because of the disease, whereas 38.5% are currently disease-free. Among patients negative for survivin expression, 100% are disease-free after a median follow-up time of 52.9 months. We did not find a significant correlation between bcl-2, box, and bcl-X gene expression and outcome of patients. In fact, these genes were found equally expressed in patients with disease progression and in disease-free patients.Conclusion: Our findings show a variable expression of apoptosis-related genes in SLNs of melanoma patients; more interestingly, we found that survivin expression correlates to outcome of patients in a statistically significant way, whereas the expression of other genes, such as bcl-2, box, and bcl-X, did not seem to correlate to progression of disease. We suggest that the detection of survivin gene expression by RT-PCR in SLNs may be a useful prognostic indicator. (C) 2003 by American Society of Clinical Oncology.