Insecticide resistance genes induce a mating competition cost in Culex pipiens mosquitoes
Insecticide resistance genes induce a mating competition cost in Culex pipiens mosquitoes
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DOI：
10.1017/s001667230100547x
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发表时间：
2002-02-01
期刊：
影响因子：
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通讯作者：
Chevillon, C
中科院分区：
文献类型：
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作者：
Berticat, C;Boquien, G;Chevillon, C
淡色库蚊对有机磷杀虫剂（OP）的抗性代表了研究抗性基因的适应性成本及其起源的便捷模型，因为自然界的环境变化和适应性基因都已明确识别。两个基因座参与了这种抗性——超级基因座 Ester 和基因座 Ace。 1 - 每个显示多个抗性等位基因。人群调查显示这些抗性基因之间甚至同一基因座的抗性等位基因之间的适应成本存在差异。为了更好地了解这种健身成本及其变异性，正在研究这些抗性基因对几种健身相关性状的影响。在这里，通过两名雄性争夺一名雌性的竞争实验，我们分析了与易感雄性和彼此相关的三种抗性等位基因——Ester(4)、Ester(1)和Ace.1(R)——对亲子成功的影响。还通过使用易感和抗性雌性研究了雌性基因型对雄性交配成功的最终影响。本实验中使用的菌株具有相同的遗传背景。易感雄性在与任何具有抗性的雄性竞争时具有交配优势，这表明该性状的抗性基因成本很高。当与易感雄性竞争时，无论雌性的基因型如何，抗性雄性的亲子鉴定成功率都没有变化。当与其他有抵抗力的雄性竞争时，除了当雌性是 Ester(1) 时，亲子鉴定成功率没有明显差异。
Resistance to organophosphorus insecticides (OP) in Culex pipiens mosquitoes represents a convenient model for investigating the fitness cost of resistance genes and its origin, since both the environmental changes in nature and the adaptive genes are clearly identified. Two loci are involved in this resistance - the super-locus Ester and the locus Ace. 1 - each displaying several resistance alleles. Population surveys have shown differences in fitness cost between these resistance genes and even between resistance alleles of the same locus. In order to better understand this fitness cost and its variability, the effects of these resistance genes on several fitness-related traits are being studied. Here, through competition experiments between two males for the access to one female, we analysed the effect on paternity success associated with three resistance alleles - Ester(4), Ester(1) and Ace.1(R) - relative to susceptible males and relative to one another. The eventual effect of female genotype on male mating success was also studied by using susceptible and resistant females. The strains used in this experiment had the same genetic background. Susceptible males had a mating advantage when competing with any of the resistant males, suggesting a substantial cost of resistance genes to this trait. When competing against susceptible males, the paternity success did not vary among resistant males, whatever the genotype of the female. When competing against other resistant males, no difference in paternity success was apparent, except when the female was Ester(1).