Protective effect of XY99-5038 on hydrogen peroxide induced cell death in cultured retinal neurons.
Protective effect of XY99-5038 on hydrogen peroxide induced cell death in cultured retinal neurons.
复制标题
XY99-5038 对培养的视网膜神经元中过氧化氢诱导的细胞死亡的保护作用。
DOI：
10.1016/s0024-3205(01)01122-5
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发表时间：
2001
期刊：
影响因子：
6.1
通讯作者：
Cao,W
中科院分区：
文献类型：
--
作者：
Xin,Y;Fong,YT;Wolf,G;Wolf,D;Cao,W
XY99-5038是由著名中医专家严欣博士提供的特定配方的制剂。该配方已被证明是有效的，没有副作用，但尚未在严格的实验室条件下进行研究。为了探讨XY99-5038对神经元变性的保护作用，我们以视网膜神经元培养为模型系统，研究了XY99-5038对过氧化氢(H_2O_2)诱导的细胞毒性的保护作用。碱性成纤维细胞生长因子是一种强有力的神经营养因子，作为对照。分离、分离0-2日龄SD大鼠视网膜，在人工无血清培养液中培养2周。采用四甲基偶氮唑盐(3，(4，5-dimethylthiazol-2-yl)2，5-diphenyl-tetrazolium)和乳酸脱氢酶(LDH)比色法检测细胞毒性，TdT介导的地高辛标记(TdT)法检测细胞死亡。H_2O_2处理可显著诱导视网膜神经元死亡。损伤前预先给予XY99-5038可显著抑制或减轻过氧化氢诱导的细胞毒性和细胞凋亡。这些效应被证明是剂量依赖的。我们的数据还表明，XY99-5038的保护作用强于碱性成纤维细胞生长因子。我们的数据表明，XY99-5038可能是治疗与氧化损伤相关的疾病的潜在药物。
XY99-5038 is a preparation from a specific formula provided by Dr. Yan Xin, a renowned Traditional Chinese Medicine specialist. This formulation has proven to be effective without side effects but has not been studied under rigorous laboratory conditions. In order to investigate the role of XY99-5038 in protecting neurons from degeneration, we used retinal neuronal culture as a model system to study the protective effects of XY99-5038 against hydrogen peroxide (H2O2) induced cytotoxicity. Basic fibroblast growth factor (bFGF), a potent neurotrophic factor, was employed as comparable agent. Retinas of 0–2 days old Sprague-Dawley rats were isolated, dissociated, and the neurons maintained for 2 weeks in a synthetic serum-free media. Cytotoxicity was determined by MTT (3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide) and LDH (Lactate dehydrogenase) assays, whereas apoptotic cell death was evaluated by the TUNEL (TdT-mediated digoxigenin-dUTP nick-end labeling) assay. Treatment with H2O2significantly induced death of retinal neurons. Pretreatment with XY99-5038 prior to insult greatly inhibited or attenuated H2O2induced cytotoxicity and apoptosis. These effects were shown to be dose-dependent. Our data also show that the protective effect of XY99-5038 is more potent than that of bFGF. Our data suggest that XY99-5038 could be a potential agent in the treatment of disorders associated with oxidative damage.