Screening of bat faeces for arthropod-borne apicomplexan protozoa: Babesia canis and Besnoitia besnoiti-like sequences from Chiroptera.
Screening of bat faeces for arthropod-borne apicomplexan protozoa: Babesia canis and Besnoitia besnoiti-like sequences from Chiroptera.
复制标题
DOI：
10.1186/s13071-015-1052-6
复制
发表时间：
2015-08-28
影响因子：
3.2
通讯作者：
Sprong H
中科院分区：
文献类型：
--
作者：
Hornok S;Estók P;Kováts D;Flaisz B;Takács N;Szőke K;Krawczyk A;Kontschán J;Gyuranecz M;Fedák A;Farkas R;Haarsma AJ;Sprong H
蝙蝠是生态流行病学上最重要的哺乳动物之一，因为它们存在于人类住区和动物饲养设施中。蝙蝠在建筑物中栖息可能会将兽医医学重要性的病原体带入家畜和人类的环境中。在这种情况下，蝙蝠长期以来一直被研究为各种病原体群的携带者。然而，尽管它们与节肢动物密切相关（无论是在它们的食物中还是作为它们的外寄生虫），但只有少数分子调查已经发表了关于它们作为媒介原生动物载体的作用。本研究的目的是弥补这种信息的匮乏。在匈牙利和荷兰共收集了221个蝙蝠粪便样本（大部分是个体）。提取DNA，并进行PCR和测序分析的节肢动物传播的apicomplexan原生动物的存在。在5份均来自匈牙利的样本中鉴定出犬巴贝虫（同源性为99 - 100%）。因为它被排除与硬蜱科特异性PCR，相关的蝙蝠消耗蜱，这些序列来自昆虫载体的Ba。犬或蝙蝠的感染。在来自荷兰的一个蝙蝠粪便样品中，扩增了与Besnoitia besnoiti具有最高（99%）同源性的序列。这些发现表明，犬巴贝斯虫病流行病学的某些方面被低估或未知，即昆虫传播的机械传播和/或蝙蝠对Ba的易感性的潜在作用。狗此外，蝙蝠需要加入到未来的研究中，以寻找Be的最终宿主。别客气
Bats are among the most eco-epidemiologically important mammals, owing to their presence in human settlements and animal keeping facilities. Roosting of bats in buildings may bring pathogens of veterinary-medical importance into the environment of domestic animals and humans. In this context bats have long been studied as carriers of various pathogen groups. However, despite their close association with arthropods (both in their food and as their ectoparasites), only a few molecular surveys have been published on their role as carriers of vector-borne protozoa. The aim of the present study was to compensate for this scarcity of information. Altogether 221 (mostly individual) bat faecal samples were collected in Hungary and the Netherlands. The DNA was extracted, and analysed with PCR and sequencing for the presence of arthropod-borne apicomplexan protozoa. Babesia canis canis (with 99-100 % homology) was identified in five samples, all from Hungary. Because it was excluded with an Ixodidae-specific PCR that the relevant bats consumed ticks, these sequences derive either from insect carriers of Ba. canis, or from the infection of bats. In one bat faecal sample from the Netherlands a sequence having the highest (99 %) homology to Besnoitia besnoiti was amplified. These findings suggest that some aspects of the epidemiology of canine babesiosis are underestimated or unknown, i.e. the potential role of insect-borne mechanical transmission and/or the susceptibility of bats to Ba. canis. In addition, bats need to be added to future studies in the quest for the final host of Be. besnoiti.