Responses of hematopoietic cells after ionizing-irradiation in anemic adult medaka (Oryzias latipes)
Responses of hematopoietic cells after ionizing-irradiation in anemic adult medaka (Oryzias latipes)
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DOI：
10.1080/09553002.2022.2110328
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发表时间：
2022-08-16
影响因子：
2.6
通讯作者：
Watanabe-Asaka，Tomomi
中科院分区：
文献类型：
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作者：
Nagata，Kento;Ohashi，Keita;Watanabe-Asaka，Tomomi
目的脊椎动物的造血组织对辐射高度敏感，数十年来一直在哺乳动物和硬骨鱼中研究电离辐射对造血的影响。本研究以日本青鳉鱼（Oryzias latipes）作为模型动物，在放射生物学方面积累了大量的知识，研究了硬骨鱼主要造血器官肾脏的辐射反应。研究了QST以及对外周血细胞和肾脏组织学的影响。结果当仅用质子束或碳离子束(15Gy)对肾脏进行局部照射时，照射后7天，受照射肾脏中的造血细胞和外周血中的细胞密度与全身用γ射线(15Gy)照射后的情况相同。这些结果表明，直接照射肾脏中的造血细胞会诱导细胞死亡和/或细胞周期停滞并停止红系细胞的供应。然后，在γ射线和质子束照射(15Gy)后，外周血中的细胞密度分别在4天和7天内恢复到对照水平，而在碳离子束照射(15Gy)后，外周血中的细胞密度没有恢复到对照水平。受照射肾脏的造血细胞在γ射线或质子束照射(15Gy)后21天内暂时减少并恢复到对照水平，而在碳离子束照射(15Gy)后没有恢复到对照水平。相反，当给予苯肼1天后对贫血青鳉进行照射时，外周血细胞密度的恢复延迟。无论是否接受γ射线照射，贫血诱导7天后肾脏中仍有大量造血细胞增殖。结论获得的结果强烈表明青鳉肾中的造血干细胞优先增殖并增加外周血细胞以消除贫血，即使它们受到高剂量照射的损伤。
PurposeHematopoietic tissues of vertebrates are highly radiation sensitive and the effects of ionizing radiation on the hematopoiesis have been studied in mammals and teleosts for decades. In this study, radiation responses in the kidney, the main hematopoietic organ in teleosts, were investigated in Japanese medaka (Oryzias latipes), which has been a model animal and a large body of knowledge has been accumulated in radiation biology.MethodsKidney, the main hematopoietic tissue of adult medaka fish, was locally irradiated using proton and carbon ion beams irradiation system of Takasaki Ion Accelerator for Advanced Radiation Application (TIARA), QST, and the effects on peripheral blood cells and histology of the kidney were investigated.ResultsWhen only kidneys were locally irradiated with proton or carbon ion beam (15 Gy), the hematopoietic cells in the irradiated kidney and cell density in the peripheral blood decreased 7 days after the irradiation in the same manner as after the whole-body irradiation with γ-rays (15 Gy). These results demonstrate that direct irradiation of the hematopoietic cells in the kidney induced cell death and/or cell cycle arrest and stopped the supply of erythroid cells. Then, the cell density in the peripheral blood recovered to the control level within 4 days and 7 days after the γ-ray and proton beam irradiation (15 Gy), respectively, while the cell density in the peripheral blood did not recover after the carbon ion beam irradiation (15 Gy). The hematopoietic cells in the irradiated kidneys temporarily decreased and recovered to the control level within 21 days after the γ-ray or proton beam irradiation (15 Gy), while it did not recover after the carbon ion beam irradiation (15 Gy). In contrast, the recovery of the cell density in the peripheral blood delayed when anemic medaka were irradiated 1 day after the administration of phenylhydrazine. With and without γ-ray irradiation, a large number of hematopoietic cells was still proliferating in the kidney 7 days after the anemia induction.ConclusionsThe results obtained strongly suggest that the hematopoietic stem cells in medaka kidney prioritize to proliferate and increase peripheral blood cells to eliminate anemia, even when they are damaged by high-dose irradiation.