CORRELATION OF ELECTROPHORETIC MOBILITIES FROM CAPILLARY ELECTROPHORESIS WITH PHYSICOCHEMICAL PROPERTIES OF PROTEINS AND PEPTIDES
CORRELATION OF ELECTROPHORETIC MOBILITIES FROM CAPILLARY ELECTROPHORESIS WITH PHYSICOCHEMICAL PROPERTIES OF PROTEINS AND PEPTIDES
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DOI：
10.1016/0003-2697(91)90379-8
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发表时间：
1991-08-15
影响因子：
2.9
通讯作者：
NIELSEN, RG
中科院分区：
文献类型：
--
作者：
RICKARD, EC;STROHL, MM;NIELSEN, RG
毛细管区带电泳法测得的电泳率与Q MW2 3有很好的相关性，其中Q是计算的电荷，Mw是分子质量。在恒定离子强度、温度和粘度的条件下，测定了33种不同的多肽和10种完整蛋白在pH 2.35、8.0和8.15的条件下的迁移率。这种关联表明，摩擦阻力与体积与质量成正比的球体的表面积成正比。电泳迁移率与理化性质的相关性将有助于阐明蛋白质和多肽混合物的最佳分离策略。
Excellent correlation was observed for the electrophoretic mobilities measured by capillary zone electrophoresis versus q MW2 3, where q is the calculated charge and MW is the molecular weight. Mobilities of a set of 33 diverse peptides from enzymatic digests and 10 intact proteins were measured for separations at pH 2.35, 8.0, and 8.15 with constant ionic strength, temperature, and viscosity. The correlation suggests that the frictional drag is proportional to the surface area of a sphere that has a volume proportional to the MW. The correlation of electrophoretic mobility with physicochemical properties will facilitate the elucidation of optimum separation strategies for protein and peptide mixtures.