ISOLOGOUS MONOCLONAL-ANTIBODIES CAN INDUCE ANTI-GBM GLOMERULONEPHRITIS IN RATS
ISOLOGOUS MONOCLONAL-ANTIBODIES CAN INDUCE ANTI-GBM GLOMERULONEPHRITIS IN RATS
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DOI：
10.1002/path.1711680211
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发表时间：
1992-10-01
影响因子：
7.3
通讯作者：
OKIGAKI, T
中科院分区：
文献类型：
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作者：
SADO, Y;KAGAWA, M;OKIGAKI, T
给WKY/NCRJ大鼠注射同种单抗(SR2、SR3)可引起抗肾小球基底膜抗体诱导的肾小球肾炎(抗GBM肾炎)。抗体来自肾炎性WKY/NCRJ大鼠脾融合的杂交瘤细胞和小鼠SP2骨髓瘤细胞。它们属于大鼠IgG2a亚类，沿肾小球和肾小管基底膜呈线性模式与大鼠肾脏结合。肾小球在注射后第1天出现组织学改变，第2天出现蛋白尿并血尿，第5天蛋白尿加重并达到平台期。这些结果表明，抗GBM肾炎甚至可以由同源单抗诱导，大鼠IgG2a亚类至少具有肾炎原性。用同源单抗建立抗GBM肾炎的实验模型，为进一步分析抗GBM肾炎的发病机制提供了可能。
Injection of isologous monoclonal antibodies (SR2, SR3) caused anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in WKY/NCrj rats. The antibodies were obtained from hybridoma cells derived from fusion of the spleen of a nephritic WKY/NCrj rat injected with rat solubilized renal basement membranes with adjuvant, and mouse SP2 myeloma cells. They belonged to the rat IgG2a subclass and bound to rat kidney in a linear pattern along the glomerular and tubular basement membranes. Histological changes in glomeruli were detected at day 1 after the injection; proteinuria with haematuria appeared on day 2; and proteinuria became severe and reached a plateau by day 5. These results demonstrate that anti-GBM nephritis can even be induced by an isologous monoclonal antibody and that the rat IgG2a subclass is at least nephritogenic. The experimental model of anti-GBM nephritis with isologous monoclonal antibodies makes it possible and easier to analyse further the mechanism of anti-GBM nephritis.