Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid
Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid
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DOI：
10.1007/s11356-015-4122-6
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发表时间：
2015-06-01
影响因子：
5.8
通讯作者：
Jiang, Hongyun
中科院分区：
文献类型：
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作者：
Feng, Lei;Zhang, Lan;Jiang, Hongyun
噻虫啉是一种新烟碱类杀虫剂，在世界范围内已广泛应用于农业生产。近年来，新烟碱类杀虫剂对非靶标生物的不良影响越来越引起人们的关注。本研究首次在OECD人工土壤上研究了噻虫啉对蚯蚓GST、CARE、CAT、SOD、POD和DNA损伤等分子生物标志物的影响。蚯蚓暴露于噻虫啉(1和3 mg/kg)7、14和28天，然后转移到清洁的OECD土壤中35、42和56天。结果表明，GST、CARE、CAT、SOD和POD活性在一个或多个采样时间受到抑制，然后在恢复过程中升高。在彗星实验中，橄榄尾矩也观察到了对E.Fetida的显著DNA损伤。这些结果表明，噻虫啉对蚯蚓有一定的毒害作用，这些生物标志物可用于评价噻虫啉对土壤生态环境的风险。
Thiacloprid, a neonicotinoid insecticide, has been used widely in agriculture worldwide. In recent years, the adverse effects of neonicotinoid insecticides on non-target organisms have attracted more and more attention. In the present study, effects of thiacloprid on molecular biomarkers (GST, CarE, CAT, SOD, POD, and DNA damage) of earthworm Eisenia fetida were investigated using the artificial OECD soil for the first time. Earthworms were exposed to thiacloprid (1 and 3 mg/kg) for 7, 14, and 28 days and then transferred to the clean OECD soil for 35, 42, and 56 days. Results showed that activities of GST, CarE, CAT, SOD, and POD are inhibited following the exposure to thiacloprid at one or more sample times and then increased during the recovery course compared with the control. Significant DNA damage to E. fetida was also observed by olive tail moments in comet assay. These results suggested that thiacloprid could have harmful effect on earthworms, and these studied biomarkers might be used in the assessment of the risk of thiacloprid to the soil ecosystem environment.