Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
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DOI：
10.1038/s41598-020-68483-4
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发表时间：
2020-07-14
影响因子：
4.6
通讯作者：
Cecconi, Daniela
中科院分区：
文献类型：
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作者：
Brandi, Jessica;Cheri, Samuele;Cecconi, Daniela
乳酸菌的局部应用(LAB)被认为是改善皮肤健康的有效方法。本工作旨在通过多学科的方法对乳杆菌属的六种乳酸菌裂解物的伤口愈合、抗炎、抗病原体和蛋白质组学作用进行表征。结果表明，供试乳酸菌的裂解产物能刺激角质形成细胞的增殖，植物乳杆菌SGL 07和唾液乳杆菌SGL 19通过诱导角质形成细胞迁移而加速角质形成细胞的再上皮化。细菌裂解物还减少角质形成细胞分泌特定的促炎介质。唾液乳杆菌活菌SGL19和发酵乳杆菌SGL10对金黄色葡萄球菌和化脓性葡萄球菌有一定的抑制作用，而短链球菌SGL12和副链球菌SGL04分别对金黄色葡萄球菌和化脓性葡萄球菌有抑制作用。被测试的乳酸菌裂解物还诱导暴露的角质形成细胞的特异性蛋白质组调节，涉及与其特定的伤口愈合和抗炎作用相关的蛋白质(如白细胞介素型增强因子结合因子2和ATP依赖的RNA解旋酶)和途径(如细胞因子、核因子-kB、Hedgehog和RUNX信号)的失调。这项研究表明了选定的乳酸菌的不同潜力，表明它们可能在未来成功地与传统疗法一起使用，以缓解皮肤病。
The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.