The presynaptic component of a cholinergic mechanism in the carotid body chemotransduction of hypoxia in the cat.
The presynaptic component of a cholinergic mechanism in the carotid body chemotransduction of hypoxia in the cat.
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猫缺氧颈动脉体化学转导中胆碱能机制的突触前成分。
DOI：
10.1007/978-1-4615-5891-0_36
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发表时间：
1996
影响因子：
--
通讯作者：
Ishizawa,Y
中科院分区：
文献类型：
--
作者：
Fitzgerald,RS;Shirahata,M;Ishizawa,Y
过去有研究表明，胆碱能阻滞剂联合或单独使用可大大降低颈动脉体对低氧克雷伯林格碳酸氢盐溶液（KRB）灌注的反应（Fitzgerald & Shirahata, 1994; Fitzgerald et al, 1995）。这些研究的操作模式均为颈上神经节的传导。即颈动脉小体的I型细胞类似于突触前神经元；含有乙酰胆碱（ACh）。事实上，在几项不同的研究中，I型细胞已被确定为乙酰胆碱的来源。合成酶胆碱乙酰转移酶（ChAT）已经定位在那里（Ballard & Jones, 1972; Wang et al, 1989）。Fidone等人（1977）在I型细胞中发现了高亲和力钠依赖性胆碱摄取转运体。他们还报道，去神经支配的颈动脉小体与完整的颈动脉小体含有同样多的乙酰胆碱（Fidone et al ., 1976），这表明颈动脉小体中神经的广泛支配和据报道存在的极少数神经节贡献了不可估量的少量乙酰胆碱。我们（Goldberg et al ., 1978）直接分析了颈动脉窦神经，发现其中没有明显的乙酰胆碱。
In the past we have shown that cholinergic blockers given together or singly could greatly reduce the carotid body’s response to a perfusion of hypoxic Krebs Ringer bicarbonate solution (KRB) (Fitzgerald & Shirahata, 1994; Fitzgerald et al, 1995). The operating model in those studies was transmission in the superior cervical ganglion. That is, the Type I cell of the carotid body was analogous to the presynaptic neuron; it contained acetylcholine (ACh). In fact the Type I cell has been identified as the source of ACh in several different studies. The synthesizing enzyme, choline acetyltransferase (ChAT), has been located there (Ballard & Jones, 1972; Wang et al, 1989). Fidone et al (1977) have located the high affinity sodium-dependent choline uptake transporter in the Type I cells. They have also reported that carotid bodies which had been denervated contained as much ACh as their intact counterparts (Fidone et al, 1976), suggesting that the extensive innervation in the carotid body and the very few ganglia reported to be there contribute an immeasurably small amount of ACh. And we (Goldberg et al, 1978) analyzed the carotid sinus nerve directly to find no appreciable amount of ACh in it.