Microbial biosynthesis of designer outer membrane vesicles.
Microbial biosynthesis of designer outer membrane vesicles.
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DOI：
10.1016/j.copbio.2014.02.018
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发表时间：
2014-10
影响因子：
7.7
通讯作者：
DeLisa, Matthew P.
中科院分区：
文献类型：
--
作者：
Baker, Jenny L.;Chen, Linxiao;Rosentha, Joseph A.;Putnam, David;DeLisa, Matthew P.
外膜囊泡(OMV)是一种由革兰氏阴性菌普遍分泌的纳米级蛋白脂质体。多年来，人们对这些生物制品的兴趣不断升级，导致对它们的组成、生产和疫苗潜力的发现。鉴于囊泡生物发生的许多步骤都是“可工程的”，现在有可能为特定的应用定制OMV。这种调整包括通过蛋白质、途径或基因组工程对产生OMV的细菌进行修饰，以特定方式改变最终的OMV产物。例如，有针对性地删除或上调与细胞膜相关的基因可以调节囊泡的产生或重塑囊泡成分的组成，如脂多糖。同样，细菌可以被重新编程，将异源表达的蛋白质整合到OMV的膜或腔室中。我们预计，在OMV工程领域的进一步研究将使专门的囊泡能够继续设计和生物合成，用于许多生物技术目的，从运送疫苗到分解纤维素底物。
Outer membrane vesicles (OMVs) are nanoscale proteoliposomes that are ubiquitously secreted by Gram-negative bacteria. Interest in these bioparticles has escalated over the years, leading to discoveries regarding their composition, production, and vaccine potential. Given that many steps in vesicle biogenesis are ‘engineerable,’ it is now possible to tailor OMVs for specific applications. Such tailoring involves modifying the OMV-producing bacterium through protein, pathway, or genome engineering in a manner that specifically alters the final OMV product. For example, targeted deletion or upregulation of genes associated with the cell envelope can modulate vesicle production or remodel the composition of vesicle components such as lipopolysaccharide. Likewise, bacteria can be reprogrammed to incorporate heterologously expressed proteins into either the membrane or lumenal compartment of OMVs. We anticipate that further research in the field of OMV engineering will enable continued design and biosynthesis of specialized vesicles for numerous biotechnological purposes ranging from the delivery of vaccines to the deconstruction of cellulosic substrates.
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