A viral protein orchestrates rice ethylene signaling to coordinate viral infection and insect vector-mediated transmission
A viral protein orchestrates rice ethylene signaling to coordinate viral infection and insect vector-mediated transmission
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病毒蛋白协调水稻乙烯信号传导以协调病毒感染和昆虫媒介介导的传播
DOI：
10.1016/j.molp.2022.01.006
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发表时间：
2022
期刊：
影响因子：
27.5
通讯作者：
Tong Zhang
中科院分区：
文献类型：
--
作者：
Yaling Zhao;Xue Cao;Weihua Zhong;Shunkang Zhou;Zhanbiao Li;Hong An;Xiahua Liu;Ruifeng Wu;Surakshya Bohora;Yan Wu;Zhenyi Liang;Jiahao Chen;Xin Yang;Guohui Zhou;Tong Zhang
节肢动物传播的病毒通过昆虫媒介快速传播，严重威胁人类健康和全球农业，南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）是由稻飞虱传播的危害最大的水稻病毒。然而，其在宿主植物中的传播和田间流行的分子机制在很大程度上是未知的。在这里，我们表明，SRBSDV编码的P6蛋白是一个关键的效应，调节水稻乙烯信号，以协调病毒感染和传播。在SRBSDV感染早期，P6与细胞质中的OsRTH 2相互作用以激活乙烯信号并增强SRBSDV增殖;这也排斥昆虫载体以减少侵染。在晚期感染中，P6进入细胞核，在那里它与乙烯信号传导的关键转录因子OsEIL 2相互作用。P6- 0 sEIL 2相互作用通过阻止0 sEIL 2的二聚化来抑制乙烯信号传导，从而通过吸引昆虫载体来促进病毒传播。总的来说，这些发现揭示了虫媒病毒调节宿主防御系统以促进病毒感染和传播的新分子机制。
Arthropod-borne viruses cause serious threats to human health and global agriculture by rapidly spreading via insect vectors.Southern rice black-streaked dwarf virus(SRBSDV) is the most damaging rice-infecting virus that is frequently transmitted by planthoppers. However, the molecular mechanisms underlying its propagation in the host plants and epidemics in the field are largely unknown. Here, we showed that the SRBSDV-encoded P6 protein is a key effector that regulates rice ethylene signaling to coordinate viral infection and transmission. In early SRBSDV infection, P6 interacts with OsRTH2 in the cytoplasm to activate ethylene signaling and enhance SRBSDV proliferation; this also repels the insect vector to reduce infestation. In late infection, P6 enters the nucleus, where it interacts with OsEIL2, a key transcription factor of ethylene signaling. The P6-OsEIL2 interaction suppresses ethylene signaling by preventing the dimerization of OsEIL2, thereby facilitating viral transmission by attracting the insect vector. Collectively, these findings reveal a novel molecular mechanism by which an arbovirus modulates the host defense system to promote viral infection and transmission.