Impact of a competitive marathon race on systemic cytokine and neutrophil responses
Impact of a competitive marathon race on systemic cytokine and neutrophil responses
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DOI：
10.1249/01.mss.0000048861.57899.04
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发表时间：
2003-02-01
期刊：
影响因子：
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通讯作者：
Sugawara, K
中科院分区：
文献类型：
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作者：
Suzuki, K;Nakaji, S;Sugawara, K
目的：研究细胞因子和中性粒细胞在力竭运动后的反应及其可能的联系，以探讨细胞因子和中性粒细胞是否参与运动相关的发病机制。方法：对10名男子马拉松运动员在42.195公里马拉松比赛前和结束后的血浆和尿样进行检测。采用酶联免疫吸附试验检测主要细胞因子和中性粒细胞活化标志物[髓过氧化物酶(MPO)和乳铁蛋白(LTF)]。体外测定了血浆对标准中性粒细胞和单核细胞鲁米诺依赖的化学发光反应的调节作用。结果：RACE引起外周血中性粒细胞增多，并伴有中性粒细胞增多和单核细胞增多。赛后血浆MPO和LTF浓度分别增加了1.8倍和1.4倍。运动后尿MPO和LTF浓度升高幅度较大，分别是运动前的12.3倍和3.5倍，提示中性粒细胞活化，肾清除超过血浆浓度升高。赛后血浆IL-6、IL-8、IL-10、粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)、单核细胞趋化蛋白-1(MCP-1)显著升高，尿IL-β、IL-6、G-CSF、M-CSF、MCP-1显著升高。血浆IL-6水平与中性粒细胞数量增加呈正相关(r=0.860.0 5，P<0.0 1)，提示IL-6介导了中性粒细胞的骨髓释放。此外，尿MPO浓度的升高与尿IL-6(r=0.868，P<0.01)和G-CSF(r=0.875，P<0.01)的升高呈正相关，提示这些细胞因子在体内促进了中性粒细胞的激活。然而，中性粒细胞和单核细胞与运动后血浆体外预孵育不能引起启动反应，这可能是运动诱导的血浆抗氧化活性增强所致。结论：尽管力竭运动后中性粒细胞和单核细胞分泌和激活多种细胞因子并发挥功能，但仍可诱导抗氧化性和抗炎性防御，防止运动性氧化应激。
Purpose: To investigate whether cytokines and neutrophils mediate exercise-related pathogenesis, we examined their responses and possible association after exhaustive exercise. Methods: Plasma and urine samples were obtained from 10 male runners before and after a 42.195-km marathon race. Major cytokines and neutrophil activation markers [myeloperoxidase (MPO) and lactoferrin (LTF)] were measured by enzyme-linked immunosorbent assays. Functional modulation of standard neutrophils and monocytes by plasma was determined in vitro on their luminol-dependent chemiluminescence responses. Results: The race induced peripheral neutrophilia accompanied by an increase in band neutrophils and monocytosis. Plasma MPO and LTF concentrations increased significantly by 1.8 and 1.4 times after the race. There was a greater increase in urine concentrations of MPO and LTF, 12.3 and 3.5 times after exercise, respectively, suggesting that neutrophil activation occurred and that renal clearance exceeded the increase in plasma concentrations. Plasma interleukin (IL)-6, IL-8, IL-10, granulocyte colony-stimulating factor (G-CSF), macrophage CSF (M-CSF), and monocyte chemotactic protein 1 (MCP-1) increased significantly after the race, and urine IL-beta, IL-6, G-CSF, M-CSF, and MCP-1 increased significantly. The plasma IL-6 responses correlated with the increases of band neutrophil count (r = 0.860, P < 0.01), suggesting IL-6-mediated bone marrow release of neutrophils. Furthermore, the increases in urine MPO concentration were correlated with increases in urine IL-6 (r = 0.868, P < 0.01) and G-CSF (r = 0.875, P < 0.01), suggesting that these cytokines promoted neutrophil activation in vivo. However, in vitro preincubation of neutrophils and monocytes with postexercise plasma could not cause priming responses, possibly because of the exercise-induced enhancement of plasma antioxidant activity. Conclusion: Although many cytokines recruiting and priming neutrophils and monocytes were secreted and functional after exhaustive exercise, overwhelming antioxidant and anti inflammatory defenses were induced, preventing exercise-induced oxidative stress.