Rosmarinus officinalis polyphenols activate cholinergic activities in PC12 cells through phosphorylation of ERK1/2
Rosmarinus officinalis polyphenols activate cholinergic activities in PC12 cells through phosphorylation of ERK1/2
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DOI：
10.1016/j.jep.2010.07.006
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发表时间：
2010-09-15
影响因子：
5.4
通讯作者：
Isoda, Hiroko
中科院分区：
文献类型：
--
作者：
El Omri, Abdelfatteh;Han, Junkyu;Isoda, Hiroko
研究的目的。本文旨在通过对迷迭香（Rosmarinus officinalis）的胆碱能活性和神经元分化的研究，阐明迷迭香的传统用途。材料和方法。用HPLC法测定了红曲霉多酚提取物对PC 12细胞活力、细胞分化、乙酰胆碱酯酶（AChE）活性、总胆碱、乙酰胆碱（ACh）和细胞外信号调节激酶（ERK 12）的影响。与神经生长因子（NGF）相比，本品来源于突尼斯半湿润和亚湿润地区。R.结果表明，丹参提取物能显著诱导PC 12细胞分化，提高ACh水平，增强AChE活性。丹参提取物半乙醇提取物和水乙醇提取物诱导的AChE活性最高，为对照组的137%。U 0126，ERK 1/2抑制剂。显著降低CA和RA对细胞分化和AChE活性的影响。CA和RA对PC 12细胞具有神经营养作用，其作用机制可能与诱导细胞分化和增强胆碱能活性有关。这些作用可能通过丝裂原活化蛋白激酶（MAPK）、ERK 1/2信号通路调节。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study. This paper aimed to elucidate the traditional use of Rosmarinus officinalis through the investigation of cholinergic activities and neuronal differentiation in rat pheochromocytoma PC12 cells These effects were examined in relation to the plant's habitat, the extraction procedure, and the major active compounds of R. officinalis.Materials and methods. Cell viability, cell differentiation, acetylcholinesterase (AChE) activity, total choline, acetylcholine (ACh) and extracellular signal-regulated kinases (ERK1/2) were determined in PC12 cells treated with extracts and HPLC-identified polyphenols of R. officinalis originated from Tunisian semi-and and subhumid area in comparison with nerve growth factor (NGF).Results. R. officinalis extracts potentiated cell differentiation and significantly enhanced AChE activity in PC12 cells The highest AChE activity was Induced by semi-and hydro-ethanolic extract (137% of control) Among HPLC-identified and screened polyphenols, carnosic acid (CA) and rosmarinic acid (RA) significantly induced cell differentiation, increased ACh level, and enhanced AChE activity in PC 12 cells. U0126, inhibitor of ERK1/2. significantly reduced CA and RA effects on cell differentiation and AChE activityConclusions: R. officinalis' CA and RA exhibited neurotrophic effects in PC12 cells through cell differentiation induction and cholinergic activities enhancement. These effects could be regulated by mitogen-activated protein kinase (MAPK), ERK1/2 signaling pathway. (C) 2010 Elsevier Ireland Ltd. All rights reserved.