Combinational use of lipid-based reagents for efficient transfection of primary fibroblasts and hepatoblasts
Combinational use of lipid-based reagents for efficient transfection of primary fibroblasts and hepatoblasts
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DOI：
10.2144/000114569
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发表时间：
2017-07-01
期刊：
影响因子：
2.7
通讯作者：
Hirooka, Yoshiki
中科院分区：
文献类型：
--
作者：
Ishiguro, Kazuhiro;Watanabe, Osamu;Hirooka, Yoshiki
市售的基于脂质的转染试剂广泛用于将DNA递送至细胞。然而，这些基于脂质的转染试剂在原代细胞中显示差的基因转移效率。在这里，我们展示了一种简单的方法，以提高基因转移效率，在原代成纤维细胞和成肝细胞使用脂质为基础的转染试剂的组合。我们的数据表明，与单独使用任一试剂相比，Lipofectamine LTX和FuGENE HD的组合使用提高了基因转移的效率，并且该组合实现了Lipofectamine LTX、FuGENE HD、TransFectin和成纤维细胞转染试剂的任何成对组合的最佳结果。
Commercially available lipid-based transfection reagents are widely used to deliver DNA to cells. However, these lipid-based transfection reagents show poor gene transfer efficiency in primary cells. Here, we demonstrate a simple method to improve gene transfer efficiency in primary fibroblasts and hepatoblasts using a combination of lipid-based transfection reagents. Our data show that combined use of Lipofectamine LTX and FuGENE HD increases the efficiency of gene transfer compared with the use of either reagent alone, and this combination achieves the best result of any pairwise combination of Lipofectamine LTX, FuGENE HD, TransFectin, and Fibroblast Transfection Reagent.