Emergence of multidrug-resistant clones of Salmonella Infantis in broiler chickens and humans in Hungary
Emergence of multidrug-resistant clones of Salmonella Infantis in broiler chickens and humans in Hungary
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DOI：
10.1093/jac/dkm249
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发表时间：
2007-09-01
影响因子：
5.2
通讯作者：
Nagy, Bela
中科院分区：
文献类型：
--
作者：
Nogrady, Noemi;Toth, Akos;Nagy, Bela
目的：婴儿沙门氏菌菌株收集，是从动物和人类来源的分离株在Hungary.Methods的特点：所有菌株进行噬菌体分型。纸片扩散法检测耐药性，PCR法检测耐药基因和1类整合子的存在。PFGE和质粒profiling.Results的菌株的遗传相关性进行了测试：大多数代表匈牙利不同地区的菌株的特点是噬菌体类型213和217和萘啶酸-链霉素-磺胺-四环素耐药类型。经XbaI PFGE指纹图谱分析，它们携带一个1类整合子，在855 bp可变区有一个aadA 1基因，一个泰特（A）基因，一个> 168 kb的质粒，其中66%代表一个遗传克隆。在匈牙利，肉鸡似乎是一个大型和几个小型多重耐药婴儿沙门氏菌克隆的储存库，可能通过鸡肉传染给人类
Objectives: The characterization of a Salmonella Infantis strain collection that was set up from isolates of animal and human origin obtained in Hungary in recent years.Methods: All isolates were phage typed. Antimicrobial resistance was tested by the disc diffusion method, while the presence of the antimicrobial resistance genes and class 1 integrons was investigated by PCR. Genetic relatedness of the isolates was tested by PFGE and plasmid profiling.Results: The majority of the isolates representing different parts of Hungary are characterized by phage types 213 and 217 and the nalidixic acid-streptomycin-sulphonamide-tetracycline resistance type. They harbour a class 1 integron with an aadA1 gene in the 855 bp variable region, a tet(A) gene, a > 168 kb plasmid and 66% of them represent one genetic clone as determined by XbaI PFGE fingerprinting.Conclusions: It seems that broiler chickens constitute a reservoir for one large and a few smaller multidrug-resistant Salmonella Infantis clones in Hungary, which might have spread to humans through chicken meat.