Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells.
Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells.
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Interleukin-2 在 HuT 78 细胞中诱导 DNA 拓扑异构酶 I 和 DNA 拓扑异构酶 II 的活性。
DOI：
10.1016/j.bbrc.2009.10.008
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发表时间：
2009
影响因子：
3.1
通讯作者：
Foglesong,PDavid
中科院分区：
文献类型：
--
作者：
Pham,Minh-Hieu;Kondapalli,Navya;Reckord,CalvinL;Foglesong,PDavid
研究了白细胞介素2对人T细胞系HuT 78中DNA拓扑异构酶I和DNA拓扑异构酶II活性的诱导作用。HuT 78细胞用1000 U白细胞介素-2/ml处理，并在24小时内制备HuT 78细胞核的提取物。定量测定提取物中DNA拓扑异构酶I和DNA拓扑异构酶II的活性。在用IL-2治疗后0.5、4和10 h，观察到三种伴随的DNA拓扑异构酶I和DNA拓扑异构酶II的比活性瞬时增加3- 11倍。这两种酶的比活性在每次短暂增加后恢复到基线值。这些结果表明，DNA拓扑异构酶I和DNA拓扑异构酶II的活性在用IL-2处理后在HuT 78细胞中高度调节。
The induction by interleukin-2 of DNA topoisomerase I and DNA topoisomerase II activities in the human T cell line HuT 78 was investigated. HuT 78 cells were treated with 1000U of interleukin-2/ml, and extracts of the HuT 78 nuclei were prepared over a 24h period. The extracts were assayed quantitatively for the activities of DNA topoisomerase I and DNA topoisomerase II. Three concomitant, transient increases of 3- to 11-fold in the specific activities of both DNA topoisomerase I and DNA topoisomerase II were observed following treatment with IL-2 at 0.5, 4, and 10h after treatment with interleukin-2. The specific activities of both enzymes returned to base-line values after each of these transient increases. These results reveal that the activities of DNA topoisomerase I and DNA topoisomerase II are highly regulated in HuT 78 cells upon treatment with IL-2.