Polysaccharide–Polynucleotide Complexes (III): A Novel Interaction between the β-1,3-Glucan Family and the Single-Stranded RNA Poly(C)
Polysaccharide–Polynucleotide Complexes (III): A Novel Interaction between the β-1,3-Glucan Family and the Single-Stranded RNA Poly(C)
复制标题
多糖-多核苷酸复合物 (III)：β-1,3-葡聚糖家族与单链 RNA Poly(C) 之间的新型相互作用
DOI：
10.1246/cl.2000.1242
复制
发表时间：
2000
影响因子：
1.6
通讯作者：
S. Shinkai
中科院分区：
文献类型：
--
作者：
Taro Kimura;Kazuya Koumoto;K. Sakurai;S. Shinkai
通过圆二色光谱的测定，考察了不同多糖与poly（C）形成复合物的能力。结果表明，香菇多糖和香菇多糖可以与poly（C）相互作用，而直链淀粉、支链淀粉、葡聚糖和凝胶多糖不能与poly（C）相互作用。这种差异清楚地表明水溶性β-1，3-葡聚糖可以与多核苷酸结合。复合物形成的分子量依赖性已经确定，至少30个葡萄糖序列长度是将大多数碱基掺入复合物所必需的。
We measured circular dichroism spectra to examine the complex-formation capability of various kinds of polysaccharides with poly(C). The results show that schizophyllan and lentinan can interact with poly(C), on the other hand, amylose, pullulan, dextran, and curdlan cannot. This difference clearly indicates that the water soluble β-1,3-glucan can bind with polynucleotides. The molecular weight dependence of the complex formation has established that at least 30 glucose sequence length is necessary to incorporate the most bases into the complex.