Ventilator associated pneumonia: quality of nonbronchoscopic bronchoalveolar ravage sample affects diagnostic yield
Ventilator associated pneumonia: quality of nonbronchoscopic bronchoalveolar ravage sample affects diagnostic yield
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DOI：
10.1034/j.1399-3003.2000.16f23.x
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发表时间：
2000-12-01
影响因子：
24.3
通讯作者：
Douthit, MM
中科院分区：
文献类型：
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作者：
Baughman, RP;Spencer, RE;Douthit, MM
呼吸治疗师获得的下呼吸道样本的可接受样本的预定义标准的重要性进行了研究，使用非支气管镜支气管肺泡灌洗（BAL）协议在重症监护室通气患者。在一年多的时间里，呼吸治疗师获得了219份样本。使用以下标准，其中115份被认为是足够的样本：60 mL的滴注体积，至少5 mt的抽吸液体，送去进行半定量培养的标本，10，000个菌落形成单位（cfu）的分类细胞计数。mL（-1）BAL。最常见的病原菌是金黄色葡萄球菌（S.金黄色葡萄球菌）（11个样本），虽然革兰氏阴性杆菌是单一的病原体在21个标本。在115个可接受的样品中，40个（35%）在> 10，000 cfu下生长大于或等于1个病原体。mL（-1）。对于80份不可接受的样本，将其送去进行适当培养，12份（15%）生长> 10，000 cfu。mL（-1）BAL。该差异具有显著性（卡方=9.44，p
The importance of predefined criteria for acceptable samples of respiratory therapists' obtained lower respiratory samples were studied, using a nonbronchoscopic bronchoalveolar lavage (BAL) protocol for ventilated patients in the intensive care unit.Therapists were instructed and asked to follow guidelines for obtaining samples. Over one year, 219 samples were obtained by respiratory therapists. Of these, 115 were considered to be adequate samples using the following criteria: 60 mL of instilled volume, at least 5 mt of fluid aspirated, specimens sent for semiquantitative culture, a differential cell count of 10,000 colony forming units (cfu). mL(-1) of BAL. The most common pathogen was Staphylococcus aureus (S. aureus) (11 samples), although Gram-negative bacilli were the single pathogen in 21 specimens. Of the 115 acceptable samples, 40 (35%) grew greater than or equal to1 pathogen at >10,000 cfu . mL(-1). For the 80 not acceptable samples which were sent for appropriate culture, 12 (15%) grew >10,000 cfu . mL(-1) BAL. This difference was significant (Chi-squared=9.44, p