BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development
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DOI：
10.1007/s00441-008-0655-z
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发表时间：
2008-09-01
影响因子：
3.6
通讯作者：
Jung, Han-Sung
中科院分区：
文献类型：
--
作者：
Hosoya, Akihiro;Kim, Ji-Youn;Jung, Han-Sung
尽管Hertwig的上皮根鞘（HERS）在形成牙根的形成中起着重要功能，但控制着自己的生长和分化的发育机制仍有旨在彻底阐明。由间充质细胞分泌的骨形态发生蛋白4（BMP4）作用于牙齿上皮，作为冠形成过程中细胞分化的调节剂。为了确定BMP4是否在牙齿上皮中特异性调节了它的发育，我们评估了小鼠摩尔根形成期间BMP4，BMP受体-IB（BMPR-IB）和BMPR-II的局部化。 HERRS细胞被证明表达BMPR-IB和BMPR-II。 BMP4阳性细胞在她周围的牙齿乳头上密集地检测到，从而表明BMP4参与了HERS的形成。在培养条件下植入了浸泡在BMP4，Noggin或磷酸盐缓冲盐水（PBS）中的珠子，并根据增殖细胞评估其长度。 12小时后，两组都表现出类似的她的发育模式，她的长度和形状与彼此非常相似。但是，在48小时后，在BMP4处理的组中观察到的她的伸长率明显短。另外，仅在Noggin和PBS处理的基团中可检测到增殖性细胞核抗原。这些发现表明，间充质表达的BMP4通过防止伸长和维持细胞增殖来调节他的发育。因此，BMP4可能被证明可作为各种组织工程应用中的根形成调节剂。
Although Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications.