Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia
Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia
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DOI：
10.1099/jmm.0.46310-0
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发表时间：
2006-08-01
影响因子：
3
通讯作者：
Savill, John
中科院分区：
文献类型：
--
作者：
Huang, Bixing;Yuan, Zheng;Savill, John
调查了澳大利亚昆士兰州141株嗜肺军团菌(SG)1分离株的19个主要毒力基因的分布和质粒的存在情况。结果表明，所检测的16个毒力基因在所有分离物中都存在，表明它们是环境和宿主细胞中分离物的生命必需基因。在水样分离株(44.2%)中发现65kb致病岛的频率高于病人分离株(2.7%)，表明65kb DNA片段可能有助于嗜肺乳杆菌在采样环境中的存活。然而，65kb片段在患者分离株中的低频率表明，致病岛可能不是嗜肺乳杆菌致病所必需的。在所研究的患者或水中分离的嗜肺性乳杆菌SG1中未检测到质粒。通过扩增片段长度多态性(AF1)检测，LVH和rtxA都与毒力优势基因AF1有关，而水中常见的毒力菌株AF16除一株rtxA外，不含LVH或rtxA基因。研究发现，以LVH和rtxA为致病标志物的聚合酶链式反应检测方法可用于确定分离物的感染潜力。
The distribution of 19 major virulence genes and the presence of plasmids were surveyed in 141 Legionella pneumophila serogroup (SG) 1 isolates from patients and water in Queensland, Australia. The results showed that 16 of the virulence genes examined were present in all isolates, suggesting that they are life-essential genes for isolates in the environment and host cells. The 65 kb pathogenicity island identified originally in strain Philadelphia-1(T) was detected more frequently in isolates from water (44.2 %) than in those from patients (2.7 %), indicating that the 65 kb DNA fragment may aid the survival of L. pneumophila in the sampled environment. However, the low frequency of the 65 kb fragment in isolates from patients suggests that the pathogenicity island may not be necessary for L. pneumophila to cause disease. Plasmids were not detected in the L. pneumophila SG1 isolates from patients or water studied. There was an association of both lvh and rtxA with the virulent and predominant genotype detected by amplified fragment length polymorphism, termed AF1, whereas the avirulent common isolate from water termed AF16 did not have lvh or rtxA genes, with the exception of one isolate with rtxA. It was found that a PCR detection test strategy with lvh and rtxA as pathogenesis markers would be useful for determining the infection potential of an isolate.