Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis
Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis
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DOI：
10.1159/000493952
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Sun, Qing
中科院分区：
文献类型：
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作者：
Qiao, Meng;Ding, Jian;Sun, Qing
背景/目的：环状rna （circRNAs）是进化保守的环状非编码rna，通过隔离（海绵）microRNAs （miRNAs）在多种疾病中发挥作用。然而，它们在牛皮癣中的作用尚不清楚。在本研究中，我们研究了circRNAs的表达并分析了它们在牛皮癣中的潜在功能。方法：采用SBC human ceRNA array V1.0检测银屑病病变和正常健康皮肤组织中circRNA的表达。使用基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析进行功能分析。使用miRNA靶标预测软件确定假定的miRNA反应元件（MREs）。通过定量实时逆转录聚合酶链反应，在银屑病病变和健康皮肤组织中验证了6个上调的环状rna。结果：共有4956个circRNAs（3016个上调，1940个下调；fold change 2, p< 0.05）在银屑病中被鉴定为差异表达。此外，在差异表达的环状rna中鉴定出4405个MREs。与正常健康皮肤组织相比，Hsa_circ_0061012在银屑病病变中表达上调。hsa_circ_0061012的前5位MREs分别是hsa-miR-7157-5p、hsa-miR-4769-3p、hsa-miR-6817-5p、hsa-miR-4310和hsa-miR-6882-3p。通过氧化石墨烯分析，研究了银屑病中5种mirna上调靶点中富集的生物学功能。氧化石墨烯分析发现，前30位的氧化石墨烯富集大部分与牛皮癣有关。结论：hsa_circ_0061012可能是银屑病的候选生物标志物。该结果为更好地理解cerna介导的银屑病基因调控提供了新的视角，并为进一步研究circRNA在银屑病中的功能提供了新的理论基础。
Background/Aims: Circular RNAs (circRNAs) are evolutionary conserved circular non-coding RNAs that play a role in several diseases by sequestering (sponging) microRNAs (miRNAs). However, their role in psoriasis remains unclear. In the present study, we investigated the expression of circRNAs and analyzed their potential functions in psoriasis. Methods: The SBC human ceRNA array V1.0 was used to analyze circRNA expression in psoriatic lesions and normal healthy skin tissues. Functional analyses were performed using Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Putative miRNA response elements (MREs) were identified using miRNA target prediction software. Six upregulated circRNAs were verified by quantitative real-time reverse transcription polymerase chain reaction in psoriatic lesions and healthy skin tissues. Results: A total of 4956 circRNAs (3016 upregulated and 1940 downregulated; fold change 2 and p< 0.05) were identified as differentially expressed in psoriasis. Furthermore, 4405 MREs were identified among the differentially expressed circRNAs. hsa_circ_0061012 was upregulated in psoriatic lesions compared with normal healthy skin tissues. The top five MREs of hsa_circ_0061012 were hsa-miR-7157-5p, hsa-miR-4769-3p, hsa-miR-6817-5p, hsa-miR-4310, and hsa-miR-6882-3p. GO analysis was carried out to investigate the biological functions enriched among the upregulated targets of five miRNAs in psoriasis. The GO analysis identified that most of top 30 of GO enrichment are related to psoriasis. Conclusion: hsa_circ_0061012 might be a candidate biomarker for psoriasis. The results provide a new perspective for a better understanding of ceRNA-mediated gene regulation in psoriasis, and provide a novel theoretical basis for further studies on the function of circRNA in psoriasis.