Pluripotency of bovine embryonic cell line derived from precompacting embryos.
Pluripotency of bovine embryonic cell line derived from precompacting embryos.
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DOI：
10.1089/15204550152475563
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发表时间：
2001-01-01
期刊：
影响因子：
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通讯作者：
First, N L
中科院分区：
文献类型：
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作者：
Mitalipova, M;Beyhan, Z;First, N L
本文报道了从8-16细胞预致密胚胎中建立的三个牛多能胚胎细胞系。两种细胞系培养10代，并经历自发分化。一个细胞系（Z2）已经连续培养超过3年并且保持未分化。这些细胞表达细胞表面标记物，这些标记物已常规用于表征其他物种中的胚胎干（ES）和胚胎生殖（EG）细胞，例如阶段特异性胚胎抗原SSEA-1、SSEA-3和SSEA-4以及c-Kit受体。在没有饲养层的情况下，这些细胞分化成各种细胞类型并形成胚状体（EBs）。当培养一段时间后，EB分化成三个EG层的衍生物-中胚层，外胚层和内胚层-其特征在于通过检测特定的细胞表面标记。我们的研究结果表明，Z2细胞系是多能的，类似于ES细胞系。据我们所知，这是第一个在培养中保持多能性超过150代的牛胚胎细胞系。
We report herein the establishment of three bovine pluripotent embryonic cell lines derived from 8-16-cell precompacting embryos. Two cell lines were cultured for 10 passages and underwent spontaneous differentiation. One cell line (Z2) has been cultured continuously for over 3 years and has remained undifferentiated. These cells express cell surface markers that have been used routinely to characterize embryonic stem (ES) and embryonic germ (EG) cells in other species such as stage-specific embryonic antigens SSEA-1, SSEA-3, and SSEA-4, and c-Kit receptor. In the absence of a feeder layer, these cells differentiated into a variety of cell types and formed embryoid bodies (EBs). When cultured for an extended period of time, EBs differentiated into derivatives of three EG layers - mesoderm, ectoderm, and endoderm - which were characterized by detection of specific cell surface markers. Our results indicate that the Z2 cell line is pluripotent and resembles an ES cell line. To our knowledge, this is the first bovine embryonic cell line that has remained pluripotent in culture for more than 150 passages.