Skin lipopolysaccharide-binding protein and IL-1β production after thermal injury
Skin lipopolysaccharide-binding protein and IL-1β production after thermal injury
复制标题
DOI：
10.1097/00004630-200021040-00010
复制
发表时间：
2000-07-01
期刊：
影响因子：
--
通讯作者：
Wang, SC
中科院分区：
文献类型：
--
作者：
Klein, RD;Su, GL;Wang, SC
在对烧伤的反应中，技能可以具有特征在于产生炎性细胞因子、募集免疫细胞、遏制入侵生物体和从伤口清除有害物质的炎性反应。脂多糖结合蛋白（LBP）是一种能够协调所有4种功能的分子;我们以前发现的证据表明，LBP是在手术伤口内产生的。由于LBP在细菌感染反应中的核心作用，以及在烧伤后的高感染率中，我们试图确定热损伤是否会影响伤口LBP的产生，从而影响宿主对细菌感染的反应。大鼠被给予烧伤或假烧伤，并在受伤后24、48和72小时被处死。对伤口标本进行细菌计数和LBP、信使（m）RNA和白细胞介素（IL）-1 β mRNA的检测。在烧伤组中，伤口LBP mRNA在24小时显著上调（P <0.05;烧伤vs假烧伤）;随后在48和72小时降低。免疫组化显示LBP蛋白在烧伤动物表皮中表达。烧伤组的细菌计数增加（P <0.05;烧伤与假烧伤），并持续上升72小时。烧伤组IL-1 β mRNA水平在各时间点均升高（P <0.05）。这些结果表明，烧伤创面LBP表达和细菌伤口计数之间呈负相关。尽管局部炎症表现为高IL-1 β水平，但在严重热损伤后未能维持局部LBP的产生可能使局部伤口易于细菌侵入。
In response to a burn injury, skill can have an inflammatory response characterized by the production of inflammatory cytokines, recruitment of immune cells, containment of invading organisms, and clearance of noxious substances from the wound. Lipopolysaccharide-binding protein (LBP) is a molecule that is capable of coordinating all 4 functions; we previously found evidence that suggested that LBP is produced within surgical wounds. Because of the central role of LBP in the response to bacterial infection, as well as in the high rate of infection after burn injuries, we sought to determine whether a thermal injury could affect wound LBP production and thereby affect host responses against bacterial infection. Rats were given either a burn or a sham burn and were killed 24, 48, and 72 hours after the injuries. Wound specimens were assayed for bacterial counts and for the presence of LBP, messenger (m)RNA, and interleukin (IL)-1 beta mRNA. Wound LBP mRNA was significantly upregulated at 24 hours in the group with burn injuries (P < .05; burn vs sham burn); this was followed by decreases at 48 and 72 hours. Immunohistochemistry showed LBP protein in the epidermis of animals with burns. Bacterial counts increased in the group with burn injuries (P < .05; burn vs sham burn) and continued to rise for 72 hours. IL-1 beta mRNA levels were elevated at all time points in the group with burn injuries (P < .05). These results suggest an inverse correlation between burn wound LBP expression and bacterial wound counts. This failure to maintain local LBP production after severe thermal injury despite localized inflammation shown by high IL-1 beta levels may predispose local wounds to bacterial invasion.