Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma.
Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma.
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阿帕替尼抑制神经胶质瘤中的细胞生长和转移，并促进替莫唑胺的抗肿瘤活性。
DOI：
10.3892/ol.2018.9355
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发表时间：
2018-11
期刊：
影响因子：
2.9
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Wang C;Jiang M;Hou H;Lin Q;Yan Z;Zhang X
阿帕替尼是一种小分子多靶点酪氨酸激酶抑制剂，广泛用于治疗各种类型的实体瘤。在本研究中，研究了阿帕替尼是否具有治疗胶质瘤的潜力。使用细胞计数试剂盒-8和集落形成测定来确定p53和EGFR突变的U251 MG神经胶质瘤细胞和野生型U-87 MG ATCC神经胶质瘤细胞的细胞活力。此外，通过流式细胞术、侵袭实验和创伤愈合实验研究胶质瘤细胞的凋亡、侵袭和迁移能力。还研究了阿帕替尼与替莫唑胺（TMZ）联合治疗胶质瘤的潜力。结果表明，阿帕替尼通过促进p53和EGFR突变和野生型胶质瘤细胞的细胞凋亡，显著抑制细胞增殖和集落形成。阿帕替尼治疗后，细胞侵袭和迁移能力显著降低。总体而言，本研究表明阿帕替尼和TMZ在胶质瘤中具有协同抗肿瘤作用，并为阿帕替尼在胶质瘤治疗中的应用提供了依据。
Apatinib, a small-molecule multi-targeted tyrosine kinase inhibitor, is widely used to treat various types of solid tumors. In the present study, it was investigated whether apatinib has therapeutic potential for glioma. Cell Counting Kit-8 and colony formation assays were utilized to determine the cell viability of p53- and EGFR-mutated U251MG glioma cells, and wild-type U-87MG ATCC glioma cells. Furthermore, apoptosis, and the invasion and migration abilities of glioma cells were investigated by flow cytometry, invasion assays and wound-healing assays. The potential of the combination of apatinib with temozolomide (TMZ) for glioma therapy was also investigated. The results demonstrate that apatinib significantly inhibited cell proliferation and colony formation through promoting cell apoptosis in p53- and EGFR-mutated and wild-type glioma cells. Cell invasion and migration abilities were notably decreased following treatment with apatinib. Overall, the present study indicates a synergistic antitumor effect of apatinib and TMZ in glioma, and presents a basis for the use of apatinib in glioma treatment.
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DOI：
10.1158/1078-0432.ccr-14-3195
发表时间：
2015-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Kim SJ;Lee HJ;Kim MS;Choi HJ;He J;Wu Q;Aldape K;Weinberg JS;Yung WK;Conrad CA;Langley RR;Lehembre F;Regenass U;Fidler IJ
通讯作者：
Fidler IJ
影响因子：
--
作者：
Huang L;Wei Y;Shen S;Shi Q;Bai J;Li J;Qin S;Yu H;Chen F
通讯作者：
Chen F
影响因子：
15.9
作者：
Xipell, Enric;Aragon, Tomas;Alonso, Marta M.
通讯作者：
Alonso, Marta M.
CGCG 成人弥漫性胶质瘤治疗临床实践指南。
影响因子：
9.7
作者：
Jiang, Tao;Mao, Ying;Wang, Qixue
通讯作者：
Wang, Qixue
影响因子：
45.3
作者：
Gilbert, Mark R.;Wang, Meihua;Mehta, Minesh P.
通讯作者：
Mehta, Minesh P.