Zebrafish ΔNp63 is a direct target of bmp signaling and encodes a transcriptional repressor blocking neural specification in the ventral ectoderm
Zebrafish ΔNp63 is a direct target of bmp signaling and encodes a transcriptional repressor blocking neural specification in the ventral ectoderm
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DOI：
10.1016/s1534-5807(02)00163-6
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发表时间：
2002-05-01
影响因子：
11.8
通讯作者：
Hammerschmidt, M
中科院分区：
文献类型：
--
作者：
Bakkers, J;Hild, M;Hammerschmidt, M
骨形态发生蛋白（Bmps）促进脊椎动物胚胎中胚层和外胚层的腹侧特化。在这里，我们确定斑马鱼DeltaNp63，编码的p53相关蛋白p63的亚型，作为外胚层特异性的BMP信号的直接转录靶。DeltaNp63本身作为一个转录抑制所需的腹规范在外胚层的原肠胚。DeltaNp63功能的丧失导致非神经外胚层减少，随后在皮肤和鳍芽形成期间表皮发育缺陷。相反，强迫DeltaNp63表达阻断神经发育并促进非神经发育，即使在没有Bmp信号的情况下。总之，DeltaNp63满足了“神经缺省模型”所假设的神经阻遏物的标准。".
Bone morphogenetic proteins (Bmps) promote ventral specification in both the mesoderm and the ectoderm of vertebrate embryos. Here we identify zebrafish DeltaNp63, encoding an isoform of the p53-related protein p63, as an ectoderm-specific direct transcriptional target of Bmp signaling. DeltaNp63 itself acts as a transcriptional repressor required for ventral specification in the ectoderm of gastrulating embryos. Loss of DeltaNp63 function leads to reduced nonneural ectoderm followed by defects in epidermal development during skin and fin bud formation. In contrast, forced DeltaNp63 expression blocks neural development and promotes nonneural development, even in the absence of Bmp signaling. Together, DeltaNp63 fulfills the criteria to be the neural repressor postulated by the "neural default model.".