IDENTIFICATION OF 23 COMPLEMENTATION GROUPS REQUIRED FOR POST-TRANSLATIONAL EVENTS IN THE YEAST SECRETORY PATHWAY
IDENTIFICATION OF 23 COMPLEMENTATION GROUPS REQUIRED FOR POST-TRANSLATIONAL EVENTS IN THE YEAST SECRETORY PATHWAY
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DOI：
10.1016/0092-8674(80)90128-2
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
SCHEKMAN, R
中科院分区：
文献类型：
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作者：
NOVICK, P;FIELD, C;SCHEKMAN, R
对分泌和细胞表面生长温度敏感的酿酒酵母突变体的细胞在非允许温度（37 ℃）下温育期间变得致密。C）。该性质允许通过在Ludox密度梯度上沉降诱变细胞来选择额外的分泌突变体。筛选来自致密细胞的集落的条件生长和转化酶和酸性磷酸酶的分泌。在37 ℃下积累异常大的转化酶细胞内库的sec突变株。C（188个突变克隆）属于23个互补组，突变等位基因的分布表明可以发现更多的互补组。C.许多突变体是热可逆的;在回到允许温度（25 ℃）时，C）分泌积累的转化酶。SEC突变细胞的EM显示，除了一个例外，膜封闭的分泌细胞器的温度依赖性积累。显然，这些结构代表了分泌和质膜组装共线的途径中的中间体。
Cells of a Saccharomyces cerevisiae mutant that is temperature-sensitive for secretion and cell surface growth become dense during incubation at the nonpermissive temperature (37.degree. C). This property allows the selection of additional secretory mutants by sedimentation of mutagenized cells on a Ludox density gradient. Colonies derived from dense cells are screened for conditional growth and secretion of invertase and acid phosphatase. The sec mutant strains that accumulate an abnormally large intracellular pool of invertase at 37.degree. C (188 mutant clones) fall into 23 complementation groups, and the distribution of mutant alleles suggests that more complementation groups could be found. Bud emergence and incorporation of a plasma membrane sulfate permease activity stop quickly after a shift to 37.degree. C. Many of the mutants are thermoreversible; upon return to the permissive temperature (25.degree. C) the accumulated invertase is secreted. EM of sec mutant cells reveals, with one exception, the temperature-dependent accumulation of membrane-enclosed secretory organelles. Apparently, these structures represent intermediates in a pathway in which secretion and plasma membrane assembly are colinear.