Whole body leucine metabolism during and after resistance exercise in fed humans.
Whole body leucine metabolism during and after resistance exercise in fed humans.
复制标题
进食的人在抗阻运动期间和之后的全身亮氨酸代谢。
DOI：
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发表时间：
1991
影响因子：
4.1
通讯作者：
Henry Schwarcz
中科院分区：
文献类型：
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作者：
M A Tarnopolsky;Stephanie A. Atkinson;J. MacDougall;B. Senor;PETER W. R. Lemon;Henry Schwarcz
采用稳定同位素方法研究了抗阻运动对亮氨酸氧化和全身蛋白质合成的影响。用L-[1- 13c]亮氨酸作为示踪剂，计算6名健康、喂养的男性运动员在1小时的循环阻力运动后的亮氨酸氧化和全身蛋白质合成。在运动前、运动中和运动后2小时进行测量，并对13CO2/12CO2呼气背景浓度和碳酸氢盐保留因子的变化进行校正。结果显示，运动后1和2h 13CO2/12CO2呼气背景浓度和运动过程中碳酸氢盐滞留因子显著（P < 0.01）增加。运动后15 min，碳酸氢盐滞留因子显著低于静止时（P < 0.05）。运动对亮氨酸氧化或通量、全身蛋白质合成或内源性亮氨酸出现率没有显著影响。我们的结论是，电路设定阻力运动不影响亮氨酸代谢的测量变量。此外，如果不考虑背景13CO2/12CO2呼吸富集和碳酸氢盐保留因子的变化，在计算抗阻运动中亮氨酸氧化和全身蛋白质合成时可能会出现较大的误差。
The effects of resistance exercise upon leucine oxidation and whole body protein synthesis were studied using stable isotope methodology. L-[1-13C]leucine was used as a tracer to calculate leucine oxidation and whole body protein synthesis in six healthy, fed, male athletes in response to a 1 h bout of circuit-set resistance exercise. The measurements were performed prior to, during, and for 2 h after exercise, and corrections were made for background 13CO2/12CO2 breath enrichment and bicarbonate retention factor changes. Results demonstrated significant (P less than 0.01) increases in the background 13CO2/12CO2 breath enrichment at 1 and 2h after exercise and in the bicarbonate retention factor (P less than 0.01) during exercise. At 15 min after exercise, the bicarbonate retention factor was significantly (P less than 0.05) lower than at rest. There were no significant effects of exercise on leucine oxidation or flux, whole body protein synthesis, or the rate of appearance of endogenous leucine. We concluded that circuit-set resistance exercise did not affect the measured variables of leucine metabolism. In addition, large errors in calculating leucine oxidation and whole body protein synthesis during resistance exercise can occur if background 13CO2/12CO2 breath enrichment and bicarbonate retention factor changes are not accounted for.