Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation
Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation
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DOI：
10.1002/dvdy.21204
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发表时间：
2007-07-01
影响因子：
2.5
通讯作者：
Wells, James M.
中科院分区：
文献类型：
--
作者：
Moore-Scott, Billie A.;Opoka, Robert;Wells, James M.
人们对内胚层胚层在肠管形成之前（小鼠胚胎日[e] 7.5-8.5）如何沿着前后（A-P）轴形成模式知之甚少，这主要是由于内胚层分子标记的缺乏。特别是，很少有基因标记产生中肠和后肠的内胚层后部区域。我们已经鉴定出在原肠胚阶段内胚层 (e7.5) 的离散域中表达的 8 个分子标记，表明在肠管形成之前内胚层中存在显着水平的模式。三个基因 Tmprss2、NM 029639 和 Dsp 在覆盖节点的假定中肠结构域中表达，该结构域的分子标记先前尚未鉴定。两个基因 Klf5 和 Epha2 在与原条相关的后内胚层中表达。这五个基因的表达在 e8.5、9.5 和 10.5 时持续存在于中肠和/或后肠中，表明这些基因是贯穿这些发育阶段的这些域的标记。我们已经鉴定了三个基因 Slc39a8、Amot 和 Dp1l1，它们在内脏内胚层的 e7.5 处表达。从 e9.5 开始，Dp1l1 在肝脏、中肠和后肠中从头表达。我们的研究结果表明，在原肠胚形成结束时，假定的中肠和后肠结构域正在分子水平上建立，比之前认为的要早，并强调了胎儿肠道形成之前内胚层模式化的重要性。发展动态 236：1997-2003，2007 年。(c) 2007 Wiley-Liss, Inc.
Little is known about how the endoderm germ layer is patterned along the anterior-posterior (A-P) axis before the formation of a gut tube (embryonic day [e] 7.5-8.5 in mouse), largely due to a paucity of molecular markers of endoderm. In particular, there are few genes that mark posterior domains of endoderm that give rise to the midgut and hindgut. We have identified 8 molecular markers that are expressed in discrete domains of the gastrula stage endoderm (e7.5), suggesting that a significant level of pattern exists in the endoderm before the formation of a gut tube. Three genes Tmprss2, NM 029639, and Dsp are expressed in a presumptive midgut domain overlying the node, a domain for which molecular markers have not previously been identified. Two genes, Klf5 and Epha2 are expressed in posterior endoderm associated with the primitive streak. Expression of these five genes persists in the midgut and/or hindgut at e8.5, 9.5 and 10.5, suggesting that these genes are markers of these domains throughout these stages of development. We have identified three genes Slc39a8, Amot, and Dp1l1, which are expressed in the visceral endoderm at e7.5. Starting at e9.5, Dp1l1 is expressed de novo in the liver, midgut, and hindgut. Our findings suggest that presumptive midgut and hindgut domains are being established at the molecular level by the end of gastrulation, earlier than previously thought, and emphasize the importance of endoderm patterning before the formation of the fetal gut. Developmental Dynamics 236:1997-2003, 2007. (c) 2007 Wiley-Liss, Inc.