Minocycline protects melanocytes against H2O2-induced cell death via JNK and p38 MAPK pathways.
Minocycline protects melanocytes against H2O2-induced cell death via JNK and p38 MAPK pathways.
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DOI：
10.3892/ijmm.22.1.9
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发表时间：
2008-07
影响因子：
5.4
通讯作者：
Xiuzu Song;A. Xu;W. Pan;Brittany Wallin;Rebecca Kivlin;Shan Lu;C. Cao;Z. Bi;Y. Wan
中科院分区：
文献类型：
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作者：
Xiuzu Song;A. Xu;W. Pan;Brittany Wallin;Rebecca Kivlin;Shan Lu;C. Cao;Z. Bi;Y. Wan
白癜风是一种获得性进行性疾病，表现为皮肤黑素细胞的选择性破坏。据报道，白癜风患者的血浆和病变皮肤中过氧化氢 (H2O2) 含量极高。高 H2O2 水平被认为是白癜风中黑色素细胞消失的原因。 JNK 和 p38 MAPK 受到氧化应激的强烈诱导，并与神经退行性疾病中的神经元损失相关。米诺环素是一种具有抗氧化活性的抗生素，能够减轻氧化应激引起的神经毒性。为了研究米诺环素是否可以挽救 H2O2 诱导的黑色素细胞凋亡，在存在或不存在米诺环素的情况下用 H2O2 处理培养的小鼠黑色素细胞 (B10BR)。我们的数据表明，H2O2 以浓度依赖性方式降低细胞活力，米诺环素可减弱这种作用。此外，H2O2 处理还可激活 B10BR 细胞中的 JNK 和 p38 MAPK 以及执行 caspase 3。米诺环素显着抑制 H2O2 诱导的 JNK、p38 MAPK 和 caspase 3 激活。总的来说，我们得出结论，米诺环素在体外可保护黑素细胞免受 H2O2 诱导的细胞凋亡。其保护作用与抑制JNK和p38 MAPK有关。我们的研究结果表明，米诺环素是一种临床耐受性良好的安全抗生素，可用于预防白癜风早期阶段的黑素细胞损失。
Vitiligo is an acquired and progressive disorder manifested by the selective destruction of melanocytes in the skin. An extremely high level of hydrogen peroxide (H2O2) in plasma as well as in lesional skin has been reported in vitiligo patients. High H2O2 level has been suggested to be responsible for the disappearance of melanocytes in vitiligo. JNK and p38 MAPK are strongly induced by oxidative stress and related to neuron loss in neurodegenerative disorders. Minocycline, an antibiotic possessing antioxidant activity, is capable of attenuating oxidative stress-induced neurotoxicity. To investigate whether minocycline rescues melanocytes from H2O2-induced apoptosis, cultured mouse melanocytes (B10BR) were treated with H2O2 in the presence or absence of minocycline. Our data showed that H2O2 decreases cell viability in a concentration-dependent manner which is attenuated by minocycline. Also, H2O2 treatment activates JNK and p38 MAPK, and executive caspase 3 in B10BR cells. Minocycline significantly inhibits H2O2-induced activation of JNK, p38 MAPK and caspase 3. Collectively, we concluded that minocycline protects melanocytes against H2O2-induced apoptosis in vitro. Its protective effect is associated with the inhibition of JNK and p38 MAPK. Our findings suggest that minocycline, a clinically well-tolerated, safe antibiotic, may be used to prevent melanocyte loss in the early stage of vitiligo.