High-Resolution Metabolomics Assessment of Military Personnel: Evaluating Analytical Strategies for Chemical Detection.
High-Resolution Metabolomics Assessment of Military Personnel: Evaluating Analytical Strategies for Chemical Detection.
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DOI：
10.1097/jom.0000000000000773
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发表时间：
2016-08
影响因子：
3.2
通讯作者：
Jones DP
中科院分区：
文献类型：
--
作者：
Liu KH;Walker DI;Uppal K;Tran V;Rohrbeck P;Mallon TM;Jones DP
使用高分辨率代谢组学（HRM）最大限度地检测血清代谢物。使用具有三个互补色谱相和四种电离模式的超高分辨率质谱法分析国防部血清储存库（DoDSR）样品。通过检测到的离子数量和与人体代谢组学数据库的准确质量匹配来评价化学覆盖率。单个HRM平台为高达58%的KEGG代谢物数据库提供了准确的质量匹配。结合两种分析方法将匹配率提高到72%，并包括大多数主要人体代谢途径和化学类别中的代谢物。检测和特征质量因分析配置而异。正负电喷雾离子化双色谱HRM为军人代谢评估提供了一种有效的通用方法。
To maximize detection of serum metabolites with high-resolution metabolomics (HRM). Department of Defense Serum Repository (DoDSR) samples were analyzed using ultra-high resolution mass spectrometry with three complementary chromatographic phases and four ionization modes. Chemical coverage was evaluated by number of ions detected and accurate mass matches to a human metabolomics database. Individual HRM platforms provided accurate mass matches for up to 58% of the KEGG metabolite database. Combining two analytical methods increased matches to 72%, and included metabolites in most major human metabolic pathways and chemical classes. Detection and feature quality varied by analytical configuration. Dual chromatography HRM with positive and negative electrospray ionization provides an effective generalized method for metabolic assessment of military personnel.