Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations
Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations
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DOI：
10.1002/elps.200406129
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发表时间：
2005-01-01
期刊：
影响因子：
2.9
通讯作者：
Osawa, M
中科院分区：
文献类型：
--
作者：
Umetsu, K;Tanaka, M;Osawa, M
我们提出了一种可靠、快速、经济的多重扩增产物长度多态性（APLP）方法，用于分析东亚人群的单倍群诊断线粒体单核苷酸多态性（mtsnp）。通过4个9倍聚合酶链反应（PCR）和随后的电泳检测编码区36个单倍群特异性mtsnp，我们可以安全地将来自日本、韩国、中国和德国8个人群的1815个个体划分为45个相关的单倍群。单倍群编码区mtSNPs的多重APLP分析不仅对分子系统发育研究特别有用，而且由于其快速和经济的性质，对大规模的关联研究也特别有用。这是第一个用于东亚人群单倍类群的编码区mtSNPs面板。
We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region mtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.