DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture
DNA Micro-Array Gene Expression Profiling of Angiogenesis in Collagen Gel Culture
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DOI：
10.4137/cmc.s532
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发表时间：
2008-01
期刊：
影响因子：
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通讯作者：
M. Akita;K. Fujita
中科院分区：
文献类型：
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作者：
M. Akita;K. Fujita
我们使用胶原凝胶培养和DNA芯片检查了血管生成相关的基因表达谱。在毛细管形成之前和之后从培养物中分离总RNA之后，进行小鼠全基因组阵列研究。超过35，000个转录本中的73个在毛细管形成后表达。大多数基因在毛细管形成前后没有表现出任何显着差异。上调表达的基因有7个：肿瘤坏死因子α诱导蛋白（Tnfaip）2、血管内皮生长因子B b（Vegfb）、促肾上腺皮质激素释放激素受体（Crhr）2、血管内皮生长因子C（Vegfc）、成纤维细胞生长因子（Fgf）6、蛋白质O岩藻糖基转移酶（Pofut）1、缺氧诱导因子1 α亚基（Hif-1a）。共鉴定出11个下调基因; ras同源基因家族成员B（Rho B）、FGF-2、血小板反应蛋白（Thbs）1、血管生成素（Ang）3、鸟嘌呤核苷酸结合蛋白α（Gna）13、肿瘤坏死因子受体超家族成员12 a（Tnfrsf 12 a）、纤溶酶原（Plg）、Elk 3、上游结合蛋白（Ubp）1、整联蛋白α V（Itgav）、促分裂原活化蛋白激酶（Mapk）14。本研究中鉴定的这些上调和下调基因提供了在心肌梗死期间和之后改善心肌基质的策略，例如功能性血管向缺血心肌的再生长。
We examined angiogenesis-related gene expression profiles using collagen gel culture and a DNA chip. After isolation of total RNA from cultures before and after capillary tube formation, a mouse whole-genome array study was performed. Seventy-three out of over 35,000 transcripts were expressed after capillary tube formation. The majority of genes did not show any significant differences between before and after capillary tube formation. However, there were 7 up-regulated genes; tumor necrosis factor alpha-induced protein (Tnfaip) 2, vascular endothelial growth factor b (Vegfb), corticotropin releasing hormone receptor (Crhr) 2, vascular endothelial growth factor C (Vegfc), fibroblast growth factor (Fgf) 6, protein O-fucosyltransferase (Pofut) 1, hypoxia inducible factor 1 alpha subunit (Hif-1a). Eleven down-regulated genes were identified; ras homolog gene family member B (RhoB), Fgf-2, Thrombospondin (Thbs) 1, Angiogenin (Ang) 3, guanine nucleotide binding protein alpha (Gna) 13, tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a), plasminogen (Plg), Elk3, upstream binding protein (Ubp) 1, integrin alpha V (Itgav), mitogen activated protein kinase (Mapk) 14. These up- and down-regulated genes identified in this study offer the strategies which improve the myocardial substrate during and following a myocardial infarction, such as the regrowth of functional blood vessels to the ischemic myocardium.