Metabolic Engineering of Escherichia coli for de Novo Production of Betaxanthins
Metabolic Engineering of Escherichia coli for de Novo Production of Betaxanthins
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用于从头生产甜菜青素的大肠杆菌代谢工程
DOI：
10.1021/acs.jafc.0c02949
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发表时间：
2020-08-05
影响因子：
6.1
通讯作者：
Zhao, Guang-Rong
中科院分区：
文献类型：
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作者：
Hou, Yanan;Liu, Xue;Zhao, Guang-Rong
甜菜色素是一种新兴的天然色素，具有高着色力和稳定性、生理活性和荧光特性，在食品、化妆品和制药工业中具有潜在的应用。包括黄色甜菜黄素和红色甜菜花青苷的甜菜色素主要局限于红花目植物中。为了扩大个别betaxanthins的可用性，在这里，我们构建了一个大肠杆菌BTA 6从头生物合成betalamic酸。以该菌株为单培养平台，通过补饲氨基酸和胺，得到14种黄色甜菜黄素和2种红色甜菜黄素。此外，我们还利用染色体工程技术构建了一株L-组氨酸高产菌株，并建立了共培养体系。通过对初始接种比例和发酵条件的优化，建立了一个稳定的共培养体系，组氨酸-β-黄素产量为287.69 mg/L。这是第一次在工程菌中从头生产甜菜黄素的报道。大肠杆菌以葡萄糖为碳源。我们的工作突出了微生物细胞工厂生产单个甜菜色素的可行性。
Betalains are emerging natural pigments with high tinctorial strength and stability, physiological activities, and fluorescent properties for potential application in food, cosmetic, and pharmaceutical industries. Betalains including yellow betaxanthins and red betacyanins are mainly restricted in the Caryophyllales plants. To expand the availability of individual betaxanthins, here, we constructed an Escherichia coli BTA6 for de novo biosynthesis of betalamic acid. Using this strain as a monoculture platform, 14 yellow and 2 red betaxanthins were produced by feeding amino acids and amines. Furthermore, we constructed an L-histidine overproducing strain using chromosome engineering to deattenuate regulation and established a coculture system. After optimization of the initial inoculation ratios and fermentation conditions, the compatible and robust coculture system produced 287.69 mg/L of histidine-betaxanthin. This is the first report on de novo production of betaxanthins in engineered E. coli using glucose as a carbon source. Our work highlights the feasibility of microbial cell factories to produce individual betalains.