THE LYSOSOMAL MEMBRANE-GLYCOPROTEINS LAMP-1 AND LAMP-2 ARE PRESENT IN MOBILIZABLE ORGANELLES, BUT ARE ABSENT FROM THE AZUROPHIL GRANULES OF HUMAN NEUTROPHILS
THE LYSOSOMAL MEMBRANE-GLYCOPROTEINS LAMP-1 AND LAMP-2 ARE PRESENT IN MOBILIZABLE ORGANELLES, BUT ARE ABSENT FROM THE AZUROPHIL GRANULES OF HUMAN NEUTROPHILS
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DOI：
10.1042/bj3110667
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发表时间：
1995-10-15
影响因子：
4.1
通讯作者：
SJOLIN, C
中科院分区：
文献类型：
--
作者：
DAHLGREN, C;CARLSSON, SR;SJOLIN, C
在人中性粒细胞中检查了存在于大多数细胞中的溶酶体膜中的高度糖基化蛋白质组的两个成员的亚细胞定位，溶酶体相关膜蛋白1和2（Lamp-1和Lamp-2）。针对纯化的Lamp-1和Lamp-2产生的抗体在中性粒细胞的免疫染色后给出了细胞质的明显颗粒染色。使用亚细胞分级分离的嗜天青和特定的颗粒从含有质膜和分泌囊泡的轻膜馏分，和Western印迹法被用来确定这些馏分中的灯的存在。结果表明，Lamp-1和Lamp-2存在于特定颗粒富集组分和轻膜组分中，但不存在于嗜天青颗粒中。分泌囊泡从质膜分离披露，轻膜灯主要存在于分泌囊泡富集级分。在吞噬过程中，Lamp-1和Lamp-2都明显集中在摄入的颗粒周围，当分泌细胞器被动员时，它们都出现在细胞表面。
The subcellular localization of two members of a highly glycosylated protein group present in lysosomal membranes in most cells, the lysosome-associated membrane proteins 1 and 2 (Lamp-1 and Lamp-2), was examined in human neutrophil granulocytes. Antibodies that were raised against purified Lamp-1 and Lamp-2 gave a distinct granular staining of the cytoplasm upon immunostaining of neutrophils. Subcellular fractionation was used to separate the azurophil and specific granules from a light-membrane fraction containing plasma membranes and secretory vesicles, and Western blotting was used to determine the presence of the Lamps in these fractions. The results show that Lamp-1 and Lamp-2 are present in the specific-granule-enriched fraction and in the light-membrane fraction, but not in the azurophil granules. Separation of secretory vesicles from plasma membranes disclosed that the light-membrane Lamps were present primarily in the secretary-vesicle-enriched fraction. During phagocytosis both Lamp-1 and Lamp-2 became markedly concentrated around the ingested particle and they both appear on the cell surface when the secretory organelles are mobilized.