Artificial synthesis of interspecific chimeras between tuber mustard (Brassica juncea) and cabbage (Brassica oleracea) and cytological analysis
Artificial synthesis of interspecific chimeras between tuber mustard (Brassica juncea) and cabbage (Brassica oleracea) and cytological analysis
复制标题
芥菜（Brassica juncea）与卷心菜（Brassica oleracea）种间嵌合体的人工合成及细胞学分析
DOI：
10.1007/s00299-006-0150-5
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发表时间：
2006-09-01
影响因子：
6.2
通讯作者：
Zhu, Xue-Yun
中科院分区：
文献类型：
--
作者：
Chen, Li-Ping;Ge, Ya-Ming;Zhu, Xue-Yun
采用离体嫁接培养的方法获得了榨菜与红甘蓝的种间嵌合体。在嫁接前，将6日龄的榨菜和红甘蓝幼苗垂直半切，用不同浓度的6-BA和NAA处理1 min，然后对称拼接。因此，扇形嵌合体最初产生的联合茎尖。榨菜和结球甘蓝垂直切片用2 mg/l 6-BA和1 mg/lNAA处理时，嵌合芽形成率最高，达6.33%。当扇形嵌合体在含1 mg/l 6-BA的MS培养基上增殖时，逐渐产生平周嵌合体和平轴嵌合体。嵌合芽在含0.1 mg/l NAA的半强度MS培养基上生根。将生根的嵌合体驯化并转移到田间进行细胞学和形态学分析。结果表明，嵌合体的气孔密度显著高于双亲，而叶绿体大小、淀粉粒大小和数量介于双亲之间。通过流式细胞术进一步分析嵌合体，结果表明它们含有两组亲本染色体。此外，嵌合体植株还具有双亲的宝贵性状。
Interspecific chimeras between tuber mustard and red cabbage were obtained by in vitro graft-culture method. Before grafting, 6-day-old seedlings of tuber mustard and red cabbage were vertically half-cut and treated with different concentrations of 6-BA and NAA for 1 min, then, they were symmetrically fit together. As a result, sectorial chimeras were initially produced from the united shoot tips. The maximum frequency of chimeral bud formation reached 6.33% when the vertical sections of tuber mustard and cabbage were treated with 2 mg/l 6-BA and 1 mg/l NAA. When sectorial chimeras were propagated on MS medium containing 1 mg/l 6-BA, periclinal and mericlinal chimeras gradually developed. Chimeral shoots were rooted on half-strength MS medium containing 0.1 mg/l NAA. The rooted chimeras were acclimatized and transferred to the field for cytological and morphological analysis. The results showed that stomata density in the chimeras was significantly higher than that of their parents, while chloroplast size, starch grain size and number were intermediate between the two parents. The chimeras were further analyzed by flow cytometry, and the results indicated that they contained both sets of parental chromosomes. Moreover, chimeral plants possessed valuable characters from the two parents.