ON THE VITAMIN Bc CONJUGATE IN YEAST.
ON THE VITAMIN Bc CONJUGATE IN YEAST.
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关于酵母中的维生素 Bc 结合物。
DOI：
10.1126/science.100.2585.36
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发表时间：
1944
期刊：
影响因子：
56.9
通讯作者：
J. J. Pfiffner
中科院分区：
文献类型：
--
作者：
S. Binkley;O. D. Bird;E. Bloom;R. Brown;D. G. Calkins;C. J. Campbell;A. D. Emmett;J. J. Pfiffner
溶液V（澄清）二氧六环和甲苯，各一半。程序（1）用刷子在待检查的粪便上涂抹一层薄薄的涂片。(2)立即用几滴溶液I覆盖涂片。将载玻片通过本生灯或酒精火焰几次，或直到固定剂开始蒸发。如有必要，准备向载玻片中添加更多溶液以防止干燥。快把腐蚀剂倒出来。(3)立即加入几滴溶液II。再次通过幻灯片通过火焰一次或两次，倾斜它来回。确保涂上足够的染色剂以覆盖涂片。粪便应该在三到四秒钟内变成深紫色。
Solution V (clearing) dioxane and toluol, half and half. PROCEDURE (1) With a brush, make a very thin smear of the fecal material to be examined.(2) Cover smear immediately with a few drops of solution I. Pass slide through a bunsen or alcohol flame a few times or until the fixative begins to steam. Be ready to add more solution to the slide if necessary to prevent drying. Quickly pour off the mordantfixative.(3) Immediately add several drops of solution II. Again pass the slide through the flame one or two times, tilting it back and forth. Be sure that enough stain is put on to keep the smear covered. The fecal material should become a dark purple color in three or four seconds.