Metabolism of 4,4'-dichlorobiphenyl by white-rot fungi Phanerochaete chrysosporium and Phanerochaete sp MZ142
Metabolism of 4,4'-dichlorobiphenyl by white-rot fungi Phanerochaete chrysosporium and Phanerochaete sp MZ142
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DOI：
10.1007/s00253-005-0303-4
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发表时间：
2006-09-01
影响因子：
5
通讯作者：
Kondo, Ryuichiro
中科院分区：
文献类型：
--
作者：
Kamei, Ichiro;Kogura, Riichiro;Kondo, Ryuichiro
对新型多氯联苯（PCB）化合物4,4′-二氯联苯（4,4′-DCB）及其代谢物进行了白腐菌Phanerochaete chrysporium和新分离的4,4′-DCB降解白腐菌MZ142的降解实验。虽然P. chrysosporium在低氮（LN）培养基中对4,4′-DCB的降解率高于马铃薯葡萄糖肉汤（PDB）培养基，但Phanerochaete sp. MZ142在低氮（LN）培养基中对4,4′-DCB的降解率高于马铃薯葡萄糖肉汤（PDB）培养基。通过添加4,4'-DCB及其代谢中间体的代谢物鉴定，阐明了4,4'-DCB的代谢途径。4,4'-DCB最初被Phanerochaete sp. MZ142代谢为2-羟基-4,4'-DCB和3-羟基-4,4'-DCB。另一方面，P. chrysosporium将4,4'-DCB转化为3-羟基-4,4'-DCB和4-羟基-3,4'-DCB，这是通过美国国立卫生研究院的4-氯转移产生的。3-羟基-4,4'-DCB转化为3-甲氧基-4,4'-DCB；4-chlorobenzoic酸;4-chlorobenzaldehyde;和4-氯苯醇与平革菌sp. MZ142或P. chrysosporium。3-羟基-4,4′-DCB在LN培养基条件下生成4-氯苯甲酸、4-氯苯甲醛和4-氯苯醇，说明参与了次生代谢。2-羟基-4,4'-DCB未甲基化。本文首次通过对中间体的表征，证明了白腐菌降解多氯联苯过程中羟基化是关键步骤。
Degradation experiment of model polychlorinated biphenyl (PCB) compound 4,4'-dichlorobiphenyl (4,4'-DCB) and its metabolites by the white-rot fungus Phanerochaete chrysosporium and newly isolated 4,4'-DCB-degrading white-rot fungus strain MZ142 was carried out. Although P. chrysosporium showed higher degradation of 4,4'-DCB in low-nitrogen (LN) medium than that in potato dextrose broth (PDB) medium, Phanerochaete sp. MZ142 showed higher degradation of 4,4'-DCB under PDB medium condition than that in LN medium. The metabolic pathway of 4,4'-DCB was elucidated by the identification of metabolites upon addition of 4,4'-DCB and its metabolic intermediates. 4,4'-DCB was initially metabolized to 2-hydroxy-4,4'-DCB and 3-hydroxy-4,4'-DCB by Phanerochaete sp. MZ142. On the other hand, P. chrysosporium transformed 4,4'-DCB to 3-hydroxy-4,4'-DCB and 4-hydroxy-3,4'-DCB produced via a National Institutes of Health shift of 4-chlorine. 3-Hydroxy-4,4'-DCB was transformed to 3-methoxy-4,4'-DCB; 4-chlorobenzoic acid; 4-chlorobenzaldehyde; and 4-chlorobenzyl alcohol in the culture with Phanerochaete sp. MZ142 or P. chrysosporium. LN medium condition was needed to form 4-chlorobenzoic acid, 4-chlorobenzaldehyde, and 4-chlorobenzyl alcohol from 3-hydroxy-4,4'-DCB, indicating the involvement of secondary metabolism. 2-Hydroxy-4,4'-DCB was not methylated. In this paper, we proved for the first time by characterization of intermediate that hydroxylation of PCB was a key step in the PCB degradation process by white-rot fungi.