Preparation of thermoresponsive cationic copolymer brush surfaces and application of the surface to separation of biomolecules
Preparation of thermoresponsive cationic copolymer brush surfaces and application of the surface to separation of biomolecules
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DOI：
10.1021/bm701427m
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Nagase, Kenichi;Kobayashi, Jun;Okano, Teruo
采用CuCl/CuCl2/Me6TREN催化体系，在25℃的2-丙醇条件下，通过表面引发原子转移自由基聚合（ATRP）法制备了聚n -异丙基丙烯酰胺(IPAAm)-co-2-（二甲氨基）甲基丙烯酸乙酯（DMAEMA）电刷接枝二氧化硅球表面。以改性二氧化硅球为固定相，用色谱分析表征了所制备的温度响应表面。与之前报道的其他阳离子水凝胶表面相比，在水流动相中腺苷核苷酸的色谱保留时间显著增加，这表明表面和核苷酸分析物之间发生了强烈的静电阳离子共聚物刷相互作用。随着色谱柱温度的升高，腺苷核苷酸的保留时间显著降低，这是由于共聚物的碱度随着温度的升高而降低。步进温度梯度从10到50℃缩短ATP保留时间。结果表明，ATRP制备的阳离子共聚物刷状表面可以通过改变水溶液温度迅速改变其静电性能。
We have prepared poly(N-isopropylacrylamide (IPAAm)-co-2-(dimethylamino)ethylmethacrylate (DMAEMA)) brush-grafted silica bead surfaces through surface-initiated atom transfer radical polymerization (ATRP) using the CuCl/CuCl2/Me6TREN catalytic system in 2-propanol at 25 degrees C for 16 h. The prepared temperature-responsive surfaces were characterized by chromatographic analysis using the modified silica beads as stationary phases. Chromatographic retention times for adenosine nucleotides in aqueous mobile phases were significantly increased compared to that previously reported for other cationic hydrogel surfaces, indicating that strong electrostatic cationic copolymer brush interactions occur between the surfaces and nucleotide analytes. Retention times for adenosine nucleotides significantly decreased with increasing column temperature, explained by the decreasing basicity in the copolymer with increasing temperature. Step-temperature gradients from 10 to 50 degrees C shorten ATP retention times. These results indicate that cationic copolymer brush surfaces prepared by ATRP can rapidly alter their electrostatic properties by changing aqueous temperature.