Exocytosis-coordinated mechanisms for tip growth underlie pollen tube growth guidance.
Exocytosis-coordinated mechanisms for tip growth underlie pollen tube growth guidance.
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DOI：
10.1038/s41467-017-01452-0
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发表时间：
2017-11-22
影响因子：
16.6
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Luo N;Yan A;Liu G;Guo J;Rong D;Kanaoka MM;Xiao Z;Xu G;Higashiyama T;Cui X;Yang Z
许多尖端生长的细胞能够对指导信号做出反应，在此期间，细胞精确地将其生长导向指导信号的来源。虽然已经确定了信号感知的几个参与者，但对在指导过程中控制生长方向的下游信号传导知之甚少。在这里，结合建模和实验研究，我们证明了拟南芥花粉管的生长指导是由控制尖端生长的信号网络。尖端定位的胞吐作用通过整合引导信号与ROP 1 Rho GT3信号传导并协调细胞内信号传导与细胞壁力学在该网络中起关键作用。该模型再现了花粉管引导的高鲁棒性和响应性，并解释了引导效率与尖端生长系统参数之间的关系。因此，我们的研究结果建立了一个胞吐协调机制的基础上的信号梯度和尖端生长和指导之间的机械联系的细胞寻路。尖端生长的细胞可以找到它们朝向吸引信号源的生长路径。在这里，使用实验数据和数学建模，Luo等人证明了尖端定位的胞吐作用可以将引导线索与Rho GT3信号传导整合，以控制拟南芥花粉管中的细胞壁力学和指导尖端生长。
Many tip-growing cells are capable of responding to guidance cues, during which cells precisely steer their growth toward the source of guidance signals. Though several players in signal perception have been identified, little is known about the downstream signaling that controls growth direction during guidance. Here, using combined modeling and experimental studies, we demonstrate that the growth guidance of Arabidopsis pollen tubes is regulated by the signaling network that controls tip growth. Tip-localized exocytosis plays a key role in this network by integrating guidance signals with the ROP1 Rho GTPase signaling and coordinating intracellular signaling with cell wall mechanics. This model reproduces the high robustness and responsiveness of pollen tube guidance and explains the connection between guidance efficiency and the parameters of the tip growth system. Hence, our findings establish an exocytosis-coordinated mechanism underlying the cellular pathfinding guided by signal gradients and the mechanistic linkage between tip growth and guidance. Tip-growing cells can find their growing path toward the source of attractive signals. Here, using experimental data and mathematical modeling, Luo et al. demonstrate that tip-localized exocytosis can integrate guidance cues with Rho GTPase signaling to control cell wall mechanics and direct tip growth in Arabidopsis pollen tubes.
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