The novel allele of the LhMYB12 gene is involved in splatter-type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.)
The novel allele of the LhMYB12 gene is involved in splatter-type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.)
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DOI：
10.1111/nph.12572
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发表时间：
2014-02-01
期刊：
影响因子：
9.4
通讯作者：
Tasaki, Keisuke
中科院分区：
文献类型：
--
作者：
Yamagishi, Masumi;Toda, Shinya;Tasaki, Keisuke
许多被子植物科在其花朵上形成空间调节的花青素斑点。亚洲杂交百合 (Lilium spp.) cv 'Latvia' 在其花被片上形成飞溅型斑点。飞溅仅仅是由于花青素色素在花被表皮中的沉积而产生的。为了确定飞溅发育是如何调节的，我们分析了花青素生物合成基因的转录，并分离和表征了飞溅色素沉着特异的R2R3-MYB基因。所有花青素生物合成基因都在花被片含有飞溅色素的区域表达，但在其他区域不表达，表明飞溅色素沉着是由生物合成基因的转录调控引起。先前表征的 LhMYB12 调节因子并不参与飞溅色素沉着，但本研究中分离出的 LhMYB12 基因的新等位基因 LhMYB12-Lat 却有助于飞溅的形成。在“拉脱维亚”和其他表达飞溅物的百合植物中，LhMYB12-Lat 优先在花被片含有飞溅物的区域转录。后代分离分析表明，LhMYB12-Lat 基因型和飞溅表型在 F-1 群体中共分离，表明 LhMYB12-Lat 决定飞溅的存在或不存在。LhMYB12-Lat 有助于飞溅的发育，但不影响全被膜色素沉着和凸起斑点色素沉着。由于其独特的序列和不同的转录谱，LhMYB12 的这个新等位基因应该是一种与飞溅斑发育特异性相关的新型 R2R3-MYB。
Many angiosperm families develop spatially regulated anthocyanin spots on their flowers. The Asiatic hybrid lily (Lilium spp.) cv 'Latvia' develops splatter-type spots on its tepals. The splatters arise simply from the deposition of anthocyanin pigments in the tepal epidermis.To determine how splatter development was regulated, we analysed the transcription of anthocyanin biosynthesis genes, and isolated and characterized an R2R3-MYB gene specific to splatter pigmentation.All anthocyanin biosynthesis genes were expressed in splatter-containing regions of tepals, but not in other regions, indicating that splatter pigmentation is caused by the transcriptional regulation of biosynthesis genes. Previously characterized LhMYB12 regulators were not involved in splatter pigmentation, but, instead, a new allele of the LhMYB12 gene, LhMYB12-Lat, isolated in this study, contributed to splatter development. In 'Latvia' and other lily plants expressing splatters, LhMYB12-Lat was preferentially transcribed in the splatter-containing region of tepals. Progeny segregation analysis showed that LhMYB12-Lat genotype and splatter phenotype were co-segregated among the F-1 population, indicating that LhMYB12-Lat determines the presence or absence of splatters.LhMYB12-Lat contributes to splatter development, but not to full-tepal pigmentation and raised spot pigmentation. As a result of its unique sequences and different transcription profiles, this new allele of LhMYB12 should be a novel R2R3-MYB specifically associating with splatter spot development.