Chemosensitivity Testing of Human Brain Tumours in Vitro
Chemosensitivity Testing of Human Brain Tumours in Vitro
复制标题
人脑肿瘤的体外化学敏感性测试
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
M. Rosenblum
中科院分区：
文献类型：
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作者：
M. Weizsäcker;M. Rosenblum
一项测定药物诱导细胞致死率的体外方法的比较表明，只有克隆源性细胞（集落形成）测定能提供可靠的、剂量依赖性的细胞kiU指数(9)。培养人类肿瘤细胞的单层和软琼脂技术的发展鼓励了这种方法的使用（3,7），但是从肿瘤活检中获得的细胞数量很少，而且这些细胞固有的低集落形成效率（CFE）给药物筛选带来了严重的限制。用于一项基于。为了最大限度地利用活检标本中的细胞，必须有大量的肿瘤细胞，这些细胞是肿瘤内细胞的代表。从5个恶性胶质瘤活检标本中提取的细胞连续培养以增加其CFE。我们比较了原始细胞和传代细胞对BCNU的反应。将这些结果与每位患者对1,3-双（2-氯乙基）1-亚硝基脲（BCNU）或1-（2-氯-乙基）-3-环己基-1-亚硝基脲（CCNU）的临床反应进行比较。
A comparison of in vitra methods available to determine drug-induced celllethality suggested that only clonogenic cell (colony formation) assays provide a reliable and dose-dependent index of cell kiU (9). The development of monolayer and soft agar techniques for culturing human tumour cells has encouraged the use of this method (3, 7), but the small numbers of cells that can be obtained from a tumour biopsy and the intrinsic low colony forming efficiency (CFE) of these cells impart severe limitations on drug screening. For an assay based on .cells from biopsy specimens to be maxima1ly useful, a large number of tumour cells that are representative of cells within the tumour must be available for chemosensitivity testing. Cells derived from five malignant glioma biopsy specimens were serially passed in culture to increase their CFE. We compared the res ponse to BCNU of original and passaged cells. These results were compared to each patient's clinical response to 1,3-bis (2-chloroethyl)1-nitrosourea (BCNU) or 1-(2-chloro ethyl)-3-cyclohexyl-1-nitrosourea (CCNU).