Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
复制标题
绿色木霉ZT05对立枯丝核菌的抑制机制
DOI：
10.3390/plants9070912
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Liang, Xu
中科院分区：
文献类型：
--
作者：
Halifu, Saiyaremu;Deng, Xun;Liang, Xu
木霉是一种广泛分布于自然界的丝状真菌。木霉菌作为一种农业害虫的生物防治剂，近年来得到了广泛的研究。本研究旨在通过对绿色木霉ZT 05与水稻立枯病菌的平行培养，了解绿色木霉ZT 05对水稻立枯病菌的抑制机理。virens ZT 05和R.索拉尼为此，我们研究了挥发性和非挥发性代谢产物的影响。virens ZT 05对红曲霉菌丝生长和酶活性的影响。solani并分析从并行培养物收集的转录组数据。T. virens ZT 05对R.索拉尼T.的菌丝体。virens ZT 05菌丝体包裹并穿透R. solani，并抑制R.索拉尼对T. virens ZT 05对R.索拉尼对T. virens ZT 05对菌丝蛋白质含量有显著影响。Solani中的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、硒依赖的谷胱甘肽过氧化物酶（GSH-Px）、可溶性蛋白和丙二醛（MDA）。研究发现，在T. virens ZT 05和R.索拉尼结果表明，在T. virens ZT 05和R.索拉尼T. virens ZT 05对R. solani，其作用机制与重寄生和寄生作用有关。
Trichoderma is a filamentous fungus that is widely distributed in nature. As a biological control agent of agricultural pests, Trichoderma species have been widely studied in recent years. This study aimed to understand the inhibitory mechanism of Trichoderma virens ZT05 on Rhizoctonia solani through the side-by-side culture of T. virens ZT05 and R. solani. To this end, we investigated the effect of volatile and nonvolatile metabolites of T. virens ZT05 on the mycelium growth and enzyme activity of R. solani and analyzed transcriptome data collected from side-by-side culture. T. virens ZT05 has a significant antagonistic effect against R. solani. The mycelium of T. virens ZT05 spirally wraps around and penetrates the mycelium of R. solani and inhibits the growth of R. solani. The volatile and nonvolatile metabolites of T. virens ZT05 have significant inhibitory effects on the growth of R. solani. The nonvolatile metabolites of T. virens ZT05 significantly affect the mycelium proteins of R. solani, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), selenium-dependent glutathione peroxidase (GSH-Px), soluble proteins, and malondialdehyde (MDA). Twenty genes associated with hyperparasitism, including extracellular proteases, oligopeptide transporters, G-protein coupled receptors (GPCRs), chitinases, glucanases, and proteases were found to be upregulated during the antagonistic process between T. virens ZT05 and R. solani. Thirty genes related to antibiosis function, including tetracycline resistance proteins, reductases, the heat shock response, the oxidative stress response, ATP-binding cassette (ABC) efflux transporters, and multidrug resistance transporters, were found to be upregulated during the side-by-side culture of T. virens ZT05 and R. solani. T. virens ZT05 has a significant inhibitory effect on R. solani, and its mechanism of action is associated with hyperparasitism and antibiosis.